Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection

Irill Ishak; Marcus G  Eales; Laila Damiati; Xiayi Liu; Josh J Jenkins; Matthew J. Dalby; Angela H Nobbs; Maxim G Ryadnov; Bo Su

doi:10.1021/acsanm.2c04913

Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection

Irill Ishak, Marcus G Eales, Laila Damiati, Xiayi Liu, Josh J Jenkins, Matthew J. Dalby, Angela H Nobbs, Maxim G Ryadnov, Bo Su^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

8 Citations (Scopus)

Abstract

Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalised onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalisation with peptide at micromolar concentrations inhibited E. coli growth on the flat surface, S. aureus growth on the NS surface, and S. epidermidis growth on both the flat and NS surface. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalised nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.

Original language	English
Pages (from-to)	2549–2559
Number of pages	11
Journal	ACS Applied Nano Materials
Volume	6
Issue number	4
Early online date	15 Feb 2023
DOIs	https://doi.org/10.1021/acsanm.2c04913
Publication status	Published - 24 Feb 2023

Bibliographical note

Funding Information:
This work received funding from the BBSRC iCASE Studentship (EGM) (1723473), MRC (MR/S010343/1), and EU (BioTUNE). Bio-TUNE project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 872869. We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains.

Funding Information:
This work received funding from the BBSRC iCASE Studentship (EGM) (1723473) and MRC (MR/S010343/1). We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains.

Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.

Keywords

titanium
implants
bacteria
nanotopography
antimicrobial peptides

Access to Document

10.1021/acsanm.2c04913Licence: CC BY

Handle.net

Persistent link

Cite this

@article{b38672e3f7414d9c92bc656a6f6d0b16,

title = "Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection",

abstract = "Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalised onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalisation with peptide at micromolar concentrations inhibited E. coli growth on the flat surface, S. aureus growth on the NS surface, and S. epidermidis growth on both the flat and NS surface. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalised nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.",

keywords = "titanium, implants, bacteria, nanotopography, antimicrobial peptides",

author = "Irill Ishak and Eales, {Marcus G} and Laila Damiati and Xiayi Liu and Jenkins, {Josh J} and Dalby, {Matthew J.} and Nobbs, {Angela H} and Ryadnov, {Maxim G} and Bo Su",

note = "Funding Information: This work received funding from the BBSRC iCASE Studentship (EGM) (1723473), MRC (MR/S010343/1), and EU (BioTUNE). Bio-TUNE project has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement no. 872869. We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains. Funding Information: This work received funding from the BBSRC iCASE Studentship (EGM) (1723473) and MRC (MR/S010343/1). We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

month = feb,

day = "24",

doi = "10.1021/acsanm.2c04913",

language = "English",

volume = "6",

pages = "2549–2559",

journal = "ACS Applied Nano Materials",

issn = "2574-0970",

publisher = "American Chemical Society",

number = "4",

}

Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection. / Ishak, Irill; Eales, Marcus G ; Damiati, Laila et al.
In: ACS Applied Nano Materials, Vol. 6, No. 4, 24.02.2023, p. 2549–2559.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection

AU - Ishak, Irill

AU - Eales, Marcus G

AU - Damiati, Laila

AU - Liu, Xiayi

AU - Jenkins, Josh J

AU - Dalby, Matthew J.

AU - Nobbs, Angela H

AU - Ryadnov, Maxim G

AU - Su, Bo

N1 - Funding Information: This work received funding from the BBSRC iCASE Studentship (EGM) (1723473), MRC (MR/S010343/1), and EU (BioTUNE). Bio-TUNE project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 872869. We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains. Funding Information: This work received funding from the BBSRC iCASE Studentship (EGM) (1723473) and MRC (MR/S010343/1). We thank Wolfson Bioimaging Facility at the University of Bristol for their help with electron microscopy. We thank DESY Hamburg and Dr. Noei Heshmat for access to XPS. We thank Professor Mat Upton (University of Plymouth) and Professor Tim Foster (Trinity College Dublin) for provision of bacterial strains. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/2/24

Y1 - 2023/2/24

N2 - Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalised onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalisation with peptide at micromolar concentrations inhibited E. coli growth on the flat surface, S. aureus growth on the NS surface, and S. epidermidis growth on both the flat and NS surface. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalised nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.

AB - Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalised onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalisation with peptide at micromolar concentrations inhibited E. coli growth on the flat surface, S. aureus growth on the NS surface, and S. epidermidis growth on both the flat and NS surface. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalised nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.

KW - titanium

KW - implants

KW - bacteria

KW - nanotopography

KW - antimicrobial peptides

U2 - 10.1021/acsanm.2c04913

DO - 10.1021/acsanm.2c04913

M3 - Article (Academic Journal)

C2 - 36875180

SN - 2574-0970

VL - 6

SP - 2549

EP - 2559

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

IS - 4

ER -

Enhanced and Stem-Cell Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection

Abstract

Bibliographical note

Keywords

Access to Document

Handle.net

Fingerprint

Cite this