Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones

H Cimarosti; E Ashikaga; N Jaafari; L Dearden; P Rubin; K.A Wilkinson; JM Henley

doi:10.1038/jcbfm.2011.146

Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones

H Cimarosti, E Ashikaga, N Jaafari, L Dearden, P Rubin, K.A Wilkinson, JM Henley

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Abstract

Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD.

Translated title of the contribution	Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones
Original language	English
Pages (from-to)	17 - 22
Number of pages	6
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	32
Issue number	1
DOIs	https://doi.org/10.1038/jcbfm.2011.146
Publication status	Published - Jan 2012

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Other: First published on-line 12th October 2011. 21989481

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title = "Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones",

abstract = "Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD.",

author = "H Cimarosti and E Ashikaga and N Jaafari and L Dearden and P Rubin and K.A Wilkinson and JM Henley",

note = "Other: First published on-line 12th October 2011. 21989481",

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AU - Cimarosti, H

AU - Ashikaga, E

AU - Jaafari, N

AU - Dearden, L

AU - Rubin, P

AU - Wilkinson, K.A

AU - Henley, JM

N1 - Other: First published on-line 12th October 2011. 21989481

PY - 2012/1

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AB - Here, we show that oxygen and glucose deprivation (OGD) causes increased small ubiquitin-like modifier (SUMO)-1 and SUMO-2/3 conjugation to substrate proteins in cultured hippocampal neurones. Surprisingly, the SUMO protease SENP-1, which removes SUMO from conjugated proteins, was also increased by OGD, suggesting that the neuronal response to OGD involves a complex interplay between SUMOylation and deSUMOylation. Importantly, decreasing global SUMOylation in cultured hippocampal neurones by overexpression of the catalytic domain of SENP-1 increased neuronal vulnerability to OGD-induced cell death. Taken together, these results suggest a neuroprotective role for neuronal SUMOylation after OGD.

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DO - 10.1038/jcbfm.2011.146

M3 - Article (Academic Journal)

C2 - 21989481

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JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

IS - 1

ER -

Enhanced SUMOylation and SENP-1 protein levels following oxygen and glucose deprivation in neurones

Abstract

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