Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Kun Huang; Fabio Parmeggiani; Helene Ledru; Kristian Hollingsworth; Jordi Mas Pons; Andrea Marchesi; Peter Both; Ashley P. Mattey; Edward Pallister; Gregory S. Bulmer; Jolanda M. van Munster; W Bruce Turnbull; Carmen Galan; Sabine L Flitsch

doi:10.1039/C9OB01089K

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Kun Huang, Fabio Parmeggiani, Helene Ledru, Kristian Hollingsworth, Jordi Mas Pons, Andrea Marchesi, Peter Both, Ashley P. Mattey, Edward Pallister, Gregory S. Bulmer, Jolanda M. van Munster, W Bruce Turnbull, Carmen Galan, Sabine L Flitsch

Research output: Contribution to journal › Article (Academic Journal) › peer-review

4 Citations (Scopus)

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Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Original language	English
Pages (from-to)	5920-5924
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	17
Early online date	30 May 2019
DOIs	https://doi.org/10.1039/C9OB01089K
Publication status	E-pub ahead of print - 30 May 2019

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10.1039/C9OB01089K

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Chemo-enzymatic Production of Specialty Glycans.
Galan, M. C.
19/10/15 → 18/04/21
Project: Research

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Huang, K., Parmeggiani, F., Ledru, H., Hollingsworth, K., Mas Pons, J., Marchesi, A., Both, P., Mattey, A. P., Pallister, E., Bulmer, G. S., van Munster, J. M., Turnbull, W. B., Galan, C., & Flitsch, S. L. (2019). Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases. Organic and Biomolecular Chemistry, 17, 5920-5924. https://doi.org/10.1039/C9OB01089K

Huang, Kun ; Parmeggiani, Fabio ; Ledru, Helene ; Hollingsworth, Kristian ; Mas Pons, Jordi ; Marchesi, Andrea ; Both, Peter ; Mattey, Ashley P. ; Pallister, Edward ; Bulmer, Gregory S. ; van Munster, Jolanda M. ; Turnbull, W Bruce ; Galan, Carmen ; Flitsch, Sabine L. / Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases. In: Organic and Biomolecular Chemistry. 2019 ; Vol. 17. pp. 5920-5924.

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title = "Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases",

abstract = "Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.",

author = "Kun Huang and Fabio Parmeggiani and Helene Ledru and Kristian Hollingsworth and {Mas Pons}, Jordi and Andrea Marchesi and Peter Both and Mattey, {Ashley P.} and Edward Pallister and Bulmer, {Gregory S.} and {van Munster}, {Jolanda M.} and Turnbull, {W Bruce} and Carmen Galan and Flitsch, {Sabine L}",

year = "2019",

month = may,

day = "30",

doi = "10.1039/C9OB01089K",

language = "English",

volume = "17",

pages = "5920--5924",

journal = "Organic and Biomolecular Chemistry",

issn = "1477-0520",

publisher = "Royal Society of Chemistry",

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Huang, K, Parmeggiani, F, Ledru, H, Hollingsworth, K, Mas Pons, J, Marchesi, A, Both, P, Mattey, AP, Pallister, E, Bulmer, GS, van Munster, JM, Turnbull, WB, Galan, C & Flitsch, SL 2019, 'Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases', Organic and Biomolecular Chemistry, vol. 17, pp. 5920-5924. https://doi.org/10.1039/C9OB01089K

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases. / Huang, Kun; Parmeggiani, Fabio; Ledru, Helene; Hollingsworth, Kristian; Mas Pons, Jordi; Marchesi, Andrea; Both, Peter; Mattey, Ashley P.; Pallister, Edward; Bulmer, Gregory S.; van Munster, Jolanda M.; Turnbull, W Bruce; Galan, Carmen; Flitsch, Sabine L.

In: Organic and Biomolecular Chemistry, Vol. 17, 30.05.2019, p. 5920-5924.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

AU - Huang, Kun

AU - Parmeggiani, Fabio

AU - Ledru, Helene

AU - Hollingsworth, Kristian

AU - Mas Pons, Jordi

AU - Marchesi, Andrea

AU - Both, Peter

AU - Mattey, Ashley P.

AU - Pallister, Edward

AU - Bulmer, Gregory S.

AU - van Munster, Jolanda M.

AU - Turnbull, W Bruce

AU - Galan, Carmen

AU - Flitsch, Sabine L

PY - 2019/5/30

Y1 - 2019/5/30

N2 - Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

AB - Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

U2 - 10.1039/C9OB01089K

DO - 10.1039/C9OB01089K

M3 - Article (Academic Journal)

VL - 17

SP - 5920

EP - 5924

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

SN - 1477-0520

ER -

Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Abstract

Access to Document

Chemo-enzymatic Production of Specialty Glycans.

Cite this