Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Kun Huang, Fabio Parmeggiani, Helene Ledru, Kristian Hollingsworth, Jordi Mas Pons, Andrea Marchesi, Peter Both, Ashley P. Mattey, Edward Pallister, Gregory S. Bulmer, Jolanda M. van Munster, W Bruce Turnbull, Carmen Galan, Sabine L Flitsch

Research output: Contribution to journalArticle (Academic Journal)peer-review

4 Citations (Scopus)
52 Downloads (Pure)

Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.
Original languageEnglish
Pages (from-to)5920-5924
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume17
Early online date30 May 2019
DOIs
Publication statusE-pub ahead of print - 30 May 2019

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