Ephrin-B2 regulates endothelial cell morphology and motility independently of Eph-receptor binding

M.L Bochenek, S Dickinson, J.W Astin, R.H Adams, C.D Nobes

Research output: Contribution to journalArticle (Academic Journal)peer-review

67 Citations (Scopus)

Abstract

The transmembrane protein ephrin-B2 regulates angiogenesis, i.e. the formation of new blood vessels through endothelial sprouting, proliferation and remodeling processes. In addition to essential roles in the embryonic vasculature, ephrin-B2 expression is upregulated in the adult at sites of neovascularization, such as tumors and wounds. Ephrins are known to bind Eph receptor family tyrosine kinases on neighboring cells and trigger bidirectional signal transduction downstream of both interacting molecules. Here we show that ephrin-B2 dynamically modulates the motility and cellular morphology of isolated endothelial cells. Even in the absence of Eph-receptor binding, ephrin-B2 stimulates repeated cycling between actomyosin-dependent cell contraction and spreading episodes, which requires the presence of the C-terminal PDZ motif. Our results show that ephrin-B2 is a potent regulator of endothelial cell behavior, and indicate that the control of cell migration and angiogenesis by ephrins might involve both receptor-dependent and receptor-independent activities.
Translated title of the contributionEphrin-B2 regulates endothelial cell morphology and motility independently of Eph-receptor binding
Original languageEnglish
Pages (from-to)1235 - 1246
Number of pages12
JournalJournal of Cell Science
Volume123
Issue number8
DOIs
Publication statusPublished - Apr 2010

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