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ER-to-Golgi trafficking of procollagen in the absence of large carriers

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)929-948
Number of pages20
JournalJournal of Cell Biology
Volume218
Issue number3
Early online date26 Dec 2018
DOIs
DateAccepted/In press - 6 Dec 2018
DateE-pub ahead of print - 26 Dec 2018
DatePublished (current) - 4 Mar 2019

Abstract

Secretion and assembly of collagen are fundamental to the function of the extracellular matrix. Defects in the assembly of a collagen matrix lead to pathologies including fibrosis and osteogenesis imperfecta. Owing to the size of fibril-forming procollagen molecules it is assumed that they are transported from the endoplasmic reticulum to the Golgi in specialized large COPII-dependent carriers. Here, analyzing endogenous procollagen and a new engineered GFP-tagged form, we show that transport to the Golgi occurs in the absence of large (>350 nm) carriers. Large GFP-positive structures were observed occasionally, but these were nondynamic, are not COPII positive, and are labeled with markers of the ER. We propose a short-loop model of COPII-dependent ER-to-Golgi traffic that, while consistent with models of ERGIC-dependent expansion of COPII carriers, does not invoke long-range trafficking of large vesicular structures. Our findings provide an important insight into the process of procollagen trafficking and reveal a short-loop pathway from the ER to the Golgi, without the use of large carriers.

    Research areas

  • Trafficking, Organelles

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Edinburgh University Press at http://jcb.rupress.org/content/early/2018/12/21/jcb.201806035 . Please refer to any applicable terms of use of the publisher.

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    Licence: CC BY-NC-SA

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