ESEM imaging of dynamic biological processes: the closure of stomatal pores

JE McGregor, AM. Donald

Research output: Contribution to journalArticle (Academic Journal)

17 Citations (Scopus)

Abstract

Historically, electron microscopy of dynamic biological processes has been impossible to achieve in real time because conventional electron microscopy requires specimen fixation, dehydration and metallic coating. The advent of the environmental scanning electron microscope removes these restrictions, allowing fully hydrated samples to be imaged in their native state. We explore the possibility of secondary electron imaging of biological systems undergoing natural morphological changes in the microscope chamber and present a proof of principle study on the closure of stomatal pores in Tradescantia andersonia leaf tissue. An imaging protocol is developed and the advantages and limitations of this high-resolution imaging technique are considered, including a discussion of potential beam damage mechanisms.
Translated title of the contributionESEM imaging of dynamic biological processes: the closure of stomatal pores
Original languageEnglish
Pages (from-to)135 - 141
JournalJournal of Microscopy
Volume239
DOIs
Publication statusPublished - Aug 2010

Bibliographical note

Publisher: The Royal Microscopical Society

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