Estimation of historical effective population size using linkage disequilibria with marker data

L J Corbin, A Y H Liu, S C Bishop, J A Woolliams

Research output: Contribution to journalArticle (Academic Journal)peer-review

98 Citations (Scopus)
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Theory hypothesizes that the rate of decline in linkage disequilibrium (LD) as a function of distance between markers, measured by r(2), can be used to estimate effective population size (N(e)) and how it varies over time. The development of high-density genotyping makes feasible the application of this theory and has provided an impetus to improve predictions. This study considers the impact of several developments on the estimation of N(e) using both simulated and equine high-density single-nucleotide polymorphism data, when N(e) is assumed to be constant a priori and when it is not. In all models, estimates of N(e) were highly sensitive to thresholds imposed upon minor allele frequency (MAF) and to a priori assumptions on the expected r(2) for adjacent markers. Where constant N(e) was assumed a priori, then estimates with the lowest mean square error were obtained with MAF thresholds between 0.05 and 0.10, adjustment of r(2) for finite sample size, estimation of a [the limit for r(2) as recombination frequency (c) approaches 0] and relating N(e) to c (1 - c/2). The findings for predicting N(e) from models allowing variable N(e) were much less clear, apart from the desirability of correcting for finite sample size, and the lack of consistency in estimating recent N(e) (<7 generations) where estimates use data with large c. The theoretical conflicts over how estimation should proceed and uncertainty over where predictions might be expected to fit well suggest that the estimation of N(e) when it varies be carried out with extreme caution.

Original languageEnglish
Pages (from-to)257-70
Number of pages14
JournalJournal of Animal Breeding and Genetics
Issue number4
Publication statusPublished - Aug 2012

Bibliographical note

© 2012 Blackwell Verlag GmbH.


  • Animals
  • Gene Frequency
  • Genetic Markers
  • Genotyping Techniques
  • Horses
  • Linkage Disequilibrium
  • Models, Genetic
  • Polymorphism, Single Nucleotide
  • Population Density


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