TY - JOUR
T1 - Ethylenediurea (EDU) mitigates the negative effects of ozone in rice
T2 - Insights into its mode of action
AU - Ashrafuzzaman, Md
AU - Haque, Zahidul
AU - Ali, Basharat
AU - Mathew, Boby
AU - Yu, Peng
AU - Hochholdinger, Frank
AU - de Abreu Neto, Joao Braga
AU - McGillen, Max R.
AU - Ensikat, Hans Jürgen
AU - Manning, William J.
AU - Frei, Michael
PY - 2018/12
Y1 - 2018/12
N2 - Monitoring of ozone damage to crops plays an increasingly important role for the food security of many developing countries. Ethylenediurea (EDU) could be a tool to assess ozone damage to vegetation on field scale, but its physiological mode of action remains unclear. This study investigated mechanisms underlying the ozone-protection effect of EDU in controlled chamber experiments. Ozone sensitive and tolerant rice genotypes were exposed to ozone (108 ppb, 7 hr day−1) and control conditions. EDU alleviated ozone effects on plant morphology, foliar symptoms, lipid peroxidation, and photosynthetic parameters in sensitive genotypes. Transcriptome profiling by RNA sequencing revealed that thousands of genes responded to ozone in a sensitive variety, but almost none responded to EDU. Significant interactions between ozone and EDU application occurred mostly in ozone responsive genes, in which up-regulation was mitigated by EDU application. Further experiments documented ozone degrading properties of EDU, as well as EDU deposits on leaf surfaces possibly related to surface protection. EDU application did not mitigate the reaction of plants to other abiotic stresses, including iron toxicity, zinc deficiency, and salinity. This study provided evidence that EDU is a surface protectant that specifically mitigates ozone stress without interfering directly with the plants' stress response systems.
AB - Monitoring of ozone damage to crops plays an increasingly important role for the food security of many developing countries. Ethylenediurea (EDU) could be a tool to assess ozone damage to vegetation on field scale, but its physiological mode of action remains unclear. This study investigated mechanisms underlying the ozone-protection effect of EDU in controlled chamber experiments. Ozone sensitive and tolerant rice genotypes were exposed to ozone (108 ppb, 7 hr day−1) and control conditions. EDU alleviated ozone effects on plant morphology, foliar symptoms, lipid peroxidation, and photosynthetic parameters in sensitive genotypes. Transcriptome profiling by RNA sequencing revealed that thousands of genes responded to ozone in a sensitive variety, but almost none responded to EDU. Significant interactions between ozone and EDU application occurred mostly in ozone responsive genes, in which up-regulation was mitigated by EDU application. Further experiments documented ozone degrading properties of EDU, as well as EDU deposits on leaf surfaces possibly related to surface protection. EDU application did not mitigate the reaction of plants to other abiotic stresses, including iron toxicity, zinc deficiency, and salinity. This study provided evidence that EDU is a surface protectant that specifically mitigates ozone stress without interfering directly with the plants' stress response systems.
KW - air pollution
KW - food security
KW - gene expression
KW - global change
KW - phenotyping
KW - RNA-Seq
KW - tolerance breeding
UR - http://www.scopus.com/inward/record.url?scp=85053448948&partnerID=8YFLogxK
U2 - 10.1111/pce.13423
DO - 10.1111/pce.13423
M3 - Article (Academic Journal)
C2 - 30107647
AN - SCOPUS:85053448948
SN - 0140-7791
VL - 41
SP - 2882
EP - 2898
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 12
ER -
