Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats

Eva Spada; Paola Galluzzo; Alessandra Torina; Guido R Loria; Roberta Perego; Francesca Grippi; Valeria Blanda; Luciana Baggiani; Alessia D'Amico; Maria G Pennisi; Chris R Helps; Richard Malik; Mark Westman; Barbara Gandolfi; Sarah Spencer; Daniela Proverbio; Séverine Tasker

doi:10.1002/vetr.2282

Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats

Eva Spada^*
, Paola Galluzzo
, Alessandra Torina
, Guido R Loria
, Roberta Perego
, Francesca Grippi
, Valeria Blanda
, Luciana Baggiani
, Alessia D'Amico
, Maria G Pennisi
, Chris R Helps
, Richard Malik
, Mark Westman
, Barbara Gandolfi
, Sarah Spencer
, Daniela Proverbio
, Séverine Tasker

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

5 Citations (Scopus)

Abstract

BACKGROUND: In humans, blood groups are associated with varying prevalence of infections. The aim of this study was to determine if associations exist between the feline AB blood group system and haemoplasma infection.

METHODS: Data from two studies were combined. In the first study, DNA samples from 131 haemoplasma-infected and 132 haemoplasma-uninfected UK cats underwent pyrosequencing to determine their blood genotype as AA, Ab or bb. In the second study, blood samples from 160 Italian cats of known blood phenotype A, B or AB underwent PCR testing for feline haemoplasma species DNA.

RESULTS: Haemoplasma infection was demonstrated in cats of all phenotypes and genotypes. A significantly higher number of Ab genotype cats tested positive for overall haemoplasma infection status (p = 0.04) and for Mycoplasma haemofelis infection (p = 0.03).

LIMITATIONS: Haemoplasma-infected Italian cats were few, possibly increasing the chance of type II error, and the presence of purebred cats in the sample population may have had a confounding effect.

CONCLUSIONS: Feline haemoplasmas do not appear to preferentially use either blood type A or B antigens as attachment sites for erythrocyte colonisation. Further investigations in a larger number of haemoplasma-infected cats of known blood phenotype are warranted to explain the association between genotype Ab and haemoplasma infection.

Original language	English
Pages (from-to)	e2282
Journal	Veterinary Record
Volume	192
Issue number	12
Early online date	12 Oct 2022
DOIs	https://doi.org/10.1002/vetr.2282
Publication status	Published - 16 Jun 2023

Bibliographical note

Keywords

Humans
Cats
Animals
Mycoplasma/genetics
Risk Factors
Mycoplasma Infections/epidemiology
Genotype
Phenotype
United Kingdom/epidemiology
Cat Diseases/epidemiology

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Spada, E., Galluzzo, P., Torina, A., Loria, G. R., Perego, R., Grippi, F., Blanda, V., Baggiani, L., D'Amico, A., Pennisi, M. G., Helps, C. R., Malik, R., Westman, M., Gandolfi, B., Spencer, S., Proverbio, D., & Tasker, S. (2023). Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats. Veterinary Record, 192(12), e2282. https://doi.org/10.1002/vetr.2282

@article{20d165c936c04d27bbd689eb08213634,

title = "Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats",

abstract = "BACKGROUND: In humans, blood groups are associated with varying prevalence of infections. The aim of this study was to determine if associations exist between the feline AB blood group system and haemoplasma infection.METHODS: Data from two studies were combined. In the first study, DNA samples from 131 haemoplasma-infected and 132 haemoplasma-uninfected UK cats underwent pyrosequencing to determine their blood genotype as AA, Ab or bb. In the second study, blood samples from 160 Italian cats of known blood phenotype A, B or AB underwent PCR testing for feline haemoplasma species DNA.RESULTS: Haemoplasma infection was demonstrated in cats of all phenotypes and genotypes. A significantly higher number of Ab genotype cats tested positive for overall haemoplasma infection status (p = 0.04) and for Mycoplasma haemofelis infection (p = 0.03).LIMITATIONS: Haemoplasma-infected Italian cats were few, possibly increasing the chance of type II error, and the presence of purebred cats in the sample population may have had a confounding effect.CONCLUSIONS: Feline haemoplasmas do not appear to preferentially use either blood type A or B antigens as attachment sites for erythrocyte colonisation. Further investigations in a larger number of haemoplasma-infected cats of known blood phenotype are warranted to explain the association between genotype Ab and haemoplasma infection.",

keywords = "Humans, Cats, Animals, Mycoplasma/genetics, Risk Factors, Mycoplasma Infections/epidemiology, Genotype, Phenotype, United Kingdom/epidemiology, Cat Diseases/epidemiology",

author = "Eva Spada and Paola Galluzzo and Alessandra Torina and Loria, \{Guido R\} and Roberta Perego and Francesca Grippi and Valeria Blanda and Luciana Baggiani and Alessia D'Amico and Pennisi, \{Maria G\} and Helps, \{Chris R\} and Richard Malik and Mark Westman and Barbara Gandolfi and Sarah Spencer and Daniela Proverbio and S{\'e}verine Tasker",

note = "{\textcopyright} 2022 The Authors. Veterinary Record published by John Wiley \& Sons Ltd on behalf of British Veterinary Association.",

year = "2023",

month = jun,

day = "16",

doi = "10.1002/vetr.2282",

language = "English",

volume = "192",

pages = "e2282",

journal = "Veterinary Record",

issn = "0042-4900",

publisher = "John Wiley and Sons Inc",

number = "12",

}

Spada, E, Galluzzo, P, Torina, A, Loria, GR, Perego, R, Grippi, F, Blanda, V, Baggiani, L, D'Amico, A, Pennisi, MG, Helps, CR, Malik, R, Westman, M, Gandolfi, B, Spencer, S, Proverbio, D & Tasker, S 2023, 'Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats', Veterinary Record, vol. 192, no. 12, pp. e2282. https://doi.org/10.1002/vetr.2282

TY - JOUR

T1 - Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats

AU - Spada, Eva

AU - Galluzzo, Paola

AU - Torina, Alessandra

AU - Loria, Guido R

AU - Perego, Roberta

AU - Grippi, Francesca

AU - Blanda, Valeria

AU - Baggiani, Luciana

AU - D'Amico, Alessia

AU - Pennisi, Maria G

AU - Helps, Chris R

AU - Malik, Richard

AU - Westman, Mark

AU - Gandolfi, Barbara

AU - Spencer, Sarah

AU - Proverbio, Daniela

AU - Tasker, Séverine

PY - 2023/6/16

Y1 - 2023/6/16

N2 - BACKGROUND: In humans, blood groups are associated with varying prevalence of infections. The aim of this study was to determine if associations exist between the feline AB blood group system and haemoplasma infection.METHODS: Data from two studies were combined. In the first study, DNA samples from 131 haemoplasma-infected and 132 haemoplasma-uninfected UK cats underwent pyrosequencing to determine their blood genotype as AA, Ab or bb. In the second study, blood samples from 160 Italian cats of known blood phenotype A, B or AB underwent PCR testing for feline haemoplasma species DNA.RESULTS: Haemoplasma infection was demonstrated in cats of all phenotypes and genotypes. A significantly higher number of Ab genotype cats tested positive for overall haemoplasma infection status (p = 0.04) and for Mycoplasma haemofelis infection (p = 0.03).LIMITATIONS: Haemoplasma-infected Italian cats were few, possibly increasing the chance of type II error, and the presence of purebred cats in the sample population may have had a confounding effect.CONCLUSIONS: Feline haemoplasmas do not appear to preferentially use either blood type A or B antigens as attachment sites for erythrocyte colonisation. Further investigations in a larger number of haemoplasma-infected cats of known blood phenotype are warranted to explain the association between genotype Ab and haemoplasma infection.

AB - BACKGROUND: In humans, blood groups are associated with varying prevalence of infections. The aim of this study was to determine if associations exist between the feline AB blood group system and haemoplasma infection.METHODS: Data from two studies were combined. In the first study, DNA samples from 131 haemoplasma-infected and 132 haemoplasma-uninfected UK cats underwent pyrosequencing to determine their blood genotype as AA, Ab or bb. In the second study, blood samples from 160 Italian cats of known blood phenotype A, B or AB underwent PCR testing for feline haemoplasma species DNA.RESULTS: Haemoplasma infection was demonstrated in cats of all phenotypes and genotypes. A significantly higher number of Ab genotype cats tested positive for overall haemoplasma infection status (p = 0.04) and for Mycoplasma haemofelis infection (p = 0.03).LIMITATIONS: Haemoplasma-infected Italian cats were few, possibly increasing the chance of type II error, and the presence of purebred cats in the sample population may have had a confounding effect.CONCLUSIONS: Feline haemoplasmas do not appear to preferentially use either blood type A or B antigens as attachment sites for erythrocyte colonisation. Further investigations in a larger number of haemoplasma-infected cats of known blood phenotype are warranted to explain the association between genotype Ab and haemoplasma infection.

KW - Humans

KW - Cats

KW - Animals

KW - Mycoplasma/genetics

KW - Risk Factors

KW - Mycoplasma Infections/epidemiology

KW - Genotype

KW - Phenotype

KW - United Kingdom/epidemiology

KW - Cat Diseases/epidemiology

U2 - 10.1002/vetr.2282

DO - 10.1002/vetr.2282

M3 - Article (Academic Journal)

C2 - 36222468

SN - 0042-4900

VL - 192

SP - e2282

JO - Veterinary Record

JF - Veterinary Record

IS - 12

ER -

Evaluating the association between blood genotype or phenotype and haemoplasma infection in UK and Italian cats

Abstract

Bibliographical note

Keywords

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