Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa

Rami Mazaraani; Peter Timms; Philip Hill; Tamaailau Suaalii-Sauni; Tavita Niupulusu; Seiuli V. A.  Temese; Liai Iosefa-Siitia; Leveti  Auvaa; Siuomatautu A. Tapelu; Maauga F.  Maauga F. Motu; Antoinette Righarts; Michael S Walsh; Luc Rombauts; John Allan; Paddy J Horner; Wilhelmina Huston

doi:10.1093/femspd/ftz031

Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa

Rami Mazaraani, Peter Timms, Philip Hill, Tamaailau Suaalii-Sauni, Tavita Niupulusu, Seiuli V. A. Temese, Liai Iosefa-Siitia, Leveti Auvaa, Siuomatautu A. Tapelu, Maauga F. Maauga F. Motu , Antoinette Righarts, Michael S Walsh, Luc Rombauts, John Allan, Paddy J Horner, Wilhelmina Huston

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Abstract

Serological assays can be used to investigate the population burden of infection and potentially sequelae from Chlamydia. We investigated the PGP3 ELISA as a sero-epidemiological tool for infection or sub-fertility in Australian and Samoan women. The PGP3 ELISA absorbance levels were compared between groups of women with infertility, fertile, and current chlamydial infections. In the Australian groups, women with chlamydial tubal factor infertility had significantly higher absorbance levels in the PGP3 ELISA compared to fertile women (P < 0.0001), but not when compared to women with current chlamydial infection (P = 0.44). In the Samoan study, where the prevalence of chlamydial infections is much higher there were significant differences in the PGP3 ELISA absorbance levels between chlamydial sub-fertile women and fertile women (P = 0.003). There was no difference between chlamydial sub-fertile women and women with a current infection (P = 0.829). The results support that the PGP3 assay is effective for sero-epidemiological analysis of burden of infection, but not for evaluation of chlamydial pathological sequelae such as infertility.

Original language	English
Article number	ftz031
Number of pages	5
Journal	Pathogens and Disease
Volume	77
Issue number	3
DOIs	https://doi.org/10.1093/femspd/ftz031
Publication status	Published - 14 Jun 2019

Keywords

Serology
sub-fertility
Chlamydia
Tubal factor infertility
ELISA
Sero-epidemiology

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10.1093/femspd/ftz031

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Mazaraani, R., Timms, P., Hill, P., Suaalii-Sauni, T., Niupulusu, T., Temese, S. V. A., Iosefa-Siitia, L., Auvaa, L., Tapelu, S. A., Maauga F. Motu , M. F., Righarts, A., Walsh, M. S., Rombauts, L., Allan, J., Horner, P. J., & Huston, W. (2019). Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa. Pathogens and Disease, 77(3), [ftz031]. https://doi.org/10.1093/femspd/ftz031

Mazaraani, Rami ; Timms, Peter ; Hill, Philip ; Suaalii-Sauni, Tamaailau ; Niupulusu, Tavita ; Temese, Seiuli V. A. ; Iosefa-Siitia, Liai ; Auvaa, Leveti ; Tapelu, Siuomatautu A. ; Maauga F. Motu , Maauga F. ; Righarts, Antoinette ; Walsh, Michael S ; Rombauts, Luc ; Allan, John ; Horner, Paddy J ; Huston, Wilhelmina. / Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa. In: Pathogens and Disease. 2019 ; Vol. 77, No. 3.

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abstract = "Serological assays can be used to investigate the population burden of infection and potentially sequelae from Chlamydia. We investigated the PGP3 ELISA as a sero-epidemiological tool for infection or sub-fertility in Australian and Samoan women. The PGP3 ELISA absorbance levels were compared between groups of women with infertility, fertile, and current chlamydial infections. In the Australian groups, women with chlamydial tubal factor infertility had significantly higher absorbance levels in the PGP3 ELISA compared to fertile women (P < 0.0001), but not when compared to women with current chlamydial infection (P = 0.44). In the Samoan study, where the prevalence of chlamydial infections is much higher there were significant differences in the PGP3 ELISA absorbance levels between chlamydial sub-fertile women and fertile women (P = 0.003). There was no difference between chlamydial sub-fertile women and women with a current infection (P = 0.829). The results support that the PGP3 assay is effective for sero-epidemiological analysis of burden of infection, but not for evaluation of chlamydial pathological sequelae such as infertility.",

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Mazaraani, R, Timms, P, Hill, P, Suaalii-Sauni, T, Niupulusu, T, Temese, SVA, Iosefa-Siitia, L, Auvaa, L, Tapelu, SA, Maauga F. Motu , MF, Righarts, A, Walsh, MS, Rombauts, L, Allan, J, Horner, PJ & Huston, W 2019, 'Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa', Pathogens and Disease, vol. 77, no. 3, ftz031. https://doi.org/10.1093/femspd/ftz031

Evaluation of a PGP3 ELISA for surveillance of the burden of Chlamydia infection in women from Australia and Samoa. / Mazaraani, Rami; Timms, Peter; Hill, Philip; Suaalii-Sauni, Tamaailau; Niupulusu, Tavita; Temese, Seiuli V. A. ; Iosefa-Siitia, Liai; Auvaa, Leveti ; Tapelu, Siuomatautu A.; Maauga F. Motu , Maauga F. ; Righarts, Antoinette; Walsh, Michael S; Rombauts, Luc; Allan, John; Horner, Paddy J; Huston, Wilhelmina.

In: Pathogens and Disease, Vol. 77, No. 3, ftz031, 14.06.2019.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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AU - Timms, Peter

AU - Hill, Philip

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AU - Niupulusu, Tavita

AU - Temese, Seiuli V. A.

AU - Iosefa-Siitia, Liai

AU - Auvaa, Leveti

AU - Tapelu, Siuomatautu A.

AU - Maauga F. Motu , Maauga F.

AU - Righarts, Antoinette

AU - Walsh, Michael S

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AU - Allan, John

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AU - Huston, Wilhelmina

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N2 - Serological assays can be used to investigate the population burden of infection and potentially sequelae from Chlamydia. We investigated the PGP3 ELISA as a sero-epidemiological tool for infection or sub-fertility in Australian and Samoan women. The PGP3 ELISA absorbance levels were compared between groups of women with infertility, fertile, and current chlamydial infections. In the Australian groups, women with chlamydial tubal factor infertility had significantly higher absorbance levels in the PGP3 ELISA compared to fertile women (P < 0.0001), but not when compared to women with current chlamydial infection (P = 0.44). In the Samoan study, where the prevalence of chlamydial infections is much higher there were significant differences in the PGP3 ELISA absorbance levels between chlamydial sub-fertile women and fertile women (P = 0.003). There was no difference between chlamydial sub-fertile women and women with a current infection (P = 0.829). The results support that the PGP3 assay is effective for sero-epidemiological analysis of burden of infection, but not for evaluation of chlamydial pathological sequelae such as infertility.

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