Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

Robert E. S. Bain, Claire Woodall, John Elliott, Benjamin F. Arnold, Rosalind Tung, Robert Morley, Martella du Preez, Jamie K. Bartram, Anthony P. Davis, Stephen W. Gundry, Stephen Pedley

Research output: Contribution to journalArticle (Academic Journal)

10 Citations (Scopus)
252 Downloads (Pure)

Abstract

The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti-Tray®. AT was compared with IDEXX Colilert-18® and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-18® for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.

Original languageEnglish
Article number0140997
Number of pages13
JournalPLoS ONE
Volume10
Issue number10
DOIs
Publication statusPublished - 23 Oct 2015

Fingerprint Dive into the research topics of 'Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters'. Together they form a unique fingerprint.

Cite this