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Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis

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Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis. / Kedward-Dixon, Helen; Barker, Emily N; Tasker, Severine; Kipar, A.; Helps, Christopher.

In: Journal of Feline Medicine and Surgery, 02.08.2019.

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@article{e59a8ce3d78e4e04834f2bb8cd6158b2,
title = "Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis",
abstract = "ObjectivesFeline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (IFNG), tumour necrosis factor alpha (TNFA) and DC-SIGN (CD209) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n=22; non-FIP, n=10) or clinically healthy cats over 11 years of age (n=3). MethodsDNA was extracted from tissue (n=32) or blood (n=3) and PCR performed for regions of IFNG, TNFA and CD209. PCR amplicons were sequenced, each SNP genotype determined, and genotype/allele frequency for each SNP and FIP status compared. ResultsNo significant association was found between genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both IFNG g.401 and g.408 to be associated with FIP (P=0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at IFNG g.428 to be associated with FIP (P=0.06), although most cats with FIP had the CC genotype at this locus. No associations were found between any allele at TNFA g.-421, CD209 g.1900, CD209 g.2276, CD209 g.2392 and CD209 g.2713 and FIP. Conclusions and relevanceThe use of the IFNG, TNFA and CD209 SNPs described to predict risk of FIP cannot currently be recommended.",
keywords = "gamma interferon, genetic risk factor, pyrosequencing, Feline coronavirus",
author = "Helen Kedward-Dixon and Barker, {Emily N} and Severine Tasker and A. Kipar and Christopher Helps",
year = "2019",
month = "8",
day = "2",
doi = "10.1177/1098612X19865637",
language = "English",
journal = "Journal of Feline Medicine and Surgery",
issn = "1098-612X",
publisher = "SAGE Publications Ltd",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Evaluation of polymorphisms in inflammatory mediator and cellular adhesion genes as risk factors for feline infectious peritonitis

AU - Kedward-Dixon, Helen

AU - Barker, Emily N

AU - Tasker, Severine

AU - Kipar, A.

AU - Helps, Christopher

PY - 2019/8/2

Y1 - 2019/8/2

N2 - ObjectivesFeline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (IFNG), tumour necrosis factor alpha (TNFA) and DC-SIGN (CD209) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n=22; non-FIP, n=10) or clinically healthy cats over 11 years of age (n=3). MethodsDNA was extracted from tissue (n=32) or blood (n=3) and PCR performed for regions of IFNG, TNFA and CD209. PCR amplicons were sequenced, each SNP genotype determined, and genotype/allele frequency for each SNP and FIP status compared. ResultsNo significant association was found between genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both IFNG g.401 and g.408 to be associated with FIP (P=0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at IFNG g.428 to be associated with FIP (P=0.06), although most cats with FIP had the CC genotype at this locus. No associations were found between any allele at TNFA g.-421, CD209 g.1900, CD209 g.2276, CD209 g.2392 and CD209 g.2713 and FIP. Conclusions and relevanceThe use of the IFNG, TNFA and CD209 SNPs described to predict risk of FIP cannot currently be recommended.

AB - ObjectivesFeline infectious peritonitis (FIP) is a high mortality infectious disease. Single nucleotide polymorphisms (SNPs) in the genes encoding interferon gamma (IFNG), tumour necrosis factor alpha (TNFA) and DC-SIGN (CD209) have been associated with increased and decreased risk of developing FIP. This study was designed to determine whether these associations were present in a UK population of pedigree cats using samples from cats euthanased with a confirmed diagnosis (FIP, n=22; non-FIP, n=10) or clinically healthy cats over 11 years of age (n=3). MethodsDNA was extracted from tissue (n=32) or blood (n=3) and PCR performed for regions of IFNG, TNFA and CD209. PCR amplicons were sequenced, each SNP genotype determined, and genotype/allele frequency for each SNP and FIP status compared. ResultsNo significant association was found between genotype and FIP status for any SNP analysed. There was a trend for the heterozygous CT genotype at both IFNG g.401 and g.408 to be associated with FIP (P=0.13), but this genotype was also found in a substantial proportion of non-FIP cats. There was also a trend for the heterozygous CT genotype at IFNG g.428 to be associated with FIP (P=0.06), although most cats with FIP had the CC genotype at this locus. No associations were found between any allele at TNFA g.-421, CD209 g.1900, CD209 g.2276, CD209 g.2392 and CD209 g.2713 and FIP. Conclusions and relevanceThe use of the IFNG, TNFA and CD209 SNPs described to predict risk of FIP cannot currently be recommended.

KW - gamma interferon

KW - genetic risk factor

KW - pyrosequencing

KW - Feline coronavirus

U2 - 10.1177/1098612X19865637

DO - 10.1177/1098612X19865637

M3 - Article

C2 - 31373532

JO - Journal of Feline Medicine and Surgery

JF - Journal of Feline Medicine and Surgery

SN - 1098-612X

ER -