TY - JOUR
T1 - Evidence of complement-mediated killing of Discocotyle sagittata (Platyhelminthes, Monogenea) oncomiracidia
AU - Rubio-Godoy, Miguel
AU - Porter, Robert
AU - Tinsley, Richard C.
PY - 2004/8
Y1 - 2004/8
N2 - Discocotyle sagittata oncomiracidia were rapidly killed when incubated in naïve plasma and immune sera from both rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta), the killing proceeding at a faster rate with blood material from the latter fish species. The lethal activity of naïve plasma and immune sera was comparable. This was abolished after incubation at 45°C for 30min and by the addition of EDTA but not EGTA supplemented with Mg2+, indicating that complement acting via the alternative pathway is responsible for the parasiticidal effect observed. Scanning electron micrographs showed varying degrees of surface disruption in larvae exposed to fish plasma, suggesting that complement acts by breaching the oncomiracidial tegument. Control (untreated) oncomiracidia showed no damage. Ultrastructural damage was more extensive in oncomiracidia exposed to brown trout plasma than to rainbow trout plasma for equal periods, suggesting that the complement cascade may be involved in mediating host susceptibility.
AB - Discocotyle sagittata oncomiracidia were rapidly killed when incubated in naïve plasma and immune sera from both rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta), the killing proceeding at a faster rate with blood material from the latter fish species. The lethal activity of naïve plasma and immune sera was comparable. This was abolished after incubation at 45°C for 30min and by the addition of EDTA but not EGTA supplemented with Mg2+, indicating that complement acting via the alternative pathway is responsible for the parasiticidal effect observed. Scanning electron micrographs showed varying degrees of surface disruption in larvae exposed to fish plasma, suggesting that complement acts by breaching the oncomiracidial tegument. Control (untreated) oncomiracidia showed no damage. Ultrastructural damage was more extensive in oncomiracidia exposed to brown trout plasma than to rainbow trout plasma for equal periods, suggesting that the complement cascade may be involved in mediating host susceptibility.
KW - Complement
KW - Discocotyle sagittata
KW - Monogenea
KW - Non-specific immunity
KW - Oncorhynchus mykiss
KW - Salmo trutta
KW - Scanning electron microscopy
KW - Trout
UR - http://www.scopus.com/inward/record.url?scp=1842706030&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2003.11.005
DO - 10.1016/j.fsi.2003.11.005
M3 - Article (Academic Journal)
C2 - 15212729
AN - SCOPUS:1842706030
SN - 1050-4648
VL - 17
SP - 95
EP - 103
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 2
ER -