As part of the SENTRY Antimicrobial Surveillance Program, an imipenem-resistant Pseudomonas aeruginosa strain (81-11963A) was isolated from the blood culture of a female neonate institutionalized at the local children's hospital in Warsaw, Poland. Cloning of an imipenem resistance determinant revealed it to be a VIM-2 metallo-beta-lactamase, but sequence analysis of DNA adjacent to blaVIM-2 revealed it to have a unique gene context. Downstream of the blaVIM-2 gene resides an aacA4 gene encoding the AAC(6')-Ib aminoglycoside acetyltransferase. The integron containing blaVIM-2 shows high similarity to that reported from In58 in France but was novel in that it possessed a gene cassette with a 59 truncated base element only 19 base pairs (bp) long, consisting of a conserved core site and an inverse core site separated by only 5 bp. This appears to be the first report of a metallo-beta-lactamase gene arising from a pathogenic strain in Eastern Europe.
|Translated title of the contribution||Evolution of an integron carrying blaVIM-2 in Eastern Europe: Report from the SENTRY Antimicrobial Surveillance Program|
|Pages (from-to)||116 - 119|
|Number of pages||4|
|Journal||Journal of Antimicrobial Chemotherapy|
|Publication status||Published - Jul 2003|
Bibliographical notePublisher: Oxford University Press
Walsh, TR., Toleman, MA., Hryniewicz, W., Bennett, PM., & Jones, RN. (2003). Evolution of an integron carrying blaVIM-2 in Eastern Europe: Report from the SENTRY Antimicrobial Surveillance Program. Journal of Antimicrobial Chemotherapy, 52, 116 - 119.