Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats

Hidefumi Waki; Emma B Hendy; Charlie C T Hindmarch; Sabine Gouraud; Marie Toward; Sergey Kasparov; David Murphy; Julian F R Paton

doi:10.1161/HYPERTENSIONAHA.112.192252

Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats

Hidefumi Waki, Emma B Hendy, Charlie C T Hindmarch, Sabine Gouraud, Marie Toward, Sergey Kasparov, David Murphy, Julian F R Paton

Research output: Contribution to journal › Article (Academic Journal) › peer-review

43 Citations (Scopus)

Abstract

Inflammation within the brain stem microvasculature has been associated with chronic cardiovascular diseases. We found that the expression of several enzymes involved in arachidonic acid-leukotriene B4 (LTB4) production was altered in nucleus tractus solitarii (NTS) of spontaneously hypertensive rat (SHR). LTB4 produced from arachidonic acid by 5-lipoxygenase is a potent chemoattractant of leukocytes. Leukotriene B4-12-hydroxydehydrogenase (LTB4-12-HD), which degrades LTB4, was downregulated in SHR rats compared with that in Wistar-Kyoto rats. Quantitative real-time PCR revealed that LTB4-12-HD was reduced by 63% and 58% in the NTS of adult SHR and prehypertensive SHR, respectively, compared with that in age-matched Wistar-Kyoto rats (n=6). 5-lipoxygenase gene expression was upregulated in the NTS of SHR (≈50%; n=6). LTB4 levels were increased in the NTS of the SHR, (17%; n=10, P

Original language	English
Pages (from-to)	194-201
Number of pages	14
Journal	Hypertension
Volume	61
Issue number	1
DOIs	https://doi.org/10.1161/HYPERTENSIONAHA.112.192252
Publication status	Published - Jan 2013

Keywords

brain stem
hypertension
inflammation
sympathetic nervous system
CALCIUM IONOPHORE A23187
ANGIOTENSIN-II
NEUROGENIC HYPERTENSION
VASCULAR INFLAMMATION
ENDOTHELIAL-CELLS
ARACHIDONIC-ACID
BRAIN
RECEPTOR
ACTIVATION
LEUKOCYTES

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1161/HYPERTENSIONAHA.112.192252

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Bilateral BBSRC-FAPESP: Behavioural and neuroendocrine mechanisms regulating hydromineral homeostasis - a lifelong perspective
Murphy, D. (Principal Investigator)
10/01/13 → 10/05/16
Project: Research
USA - Novel insights into the mechanisms of salt appetite
Murphy, D. (Principal Investigator)
22/07/12 → 22/07/16
Project: Research
Bilateral BBSRC-FAPESP: Amelioration of the autonomic imbalances of old age with exercise exploring the molecular and physiological mechanisms
Murphy, D. (Principal Investigator)
1/02/12 → 1/02/16
Project: Research

Cite this

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title = "Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats",

abstract = "Inflammation within the brain stem microvasculature has been associated with chronic cardiovascular diseases. We found that the expression of several enzymes involved in arachidonic acid-leukotriene B4 (LTB4) production was altered in nucleus tractus solitarii (NTS) of spontaneously hypertensive rat (SHR). LTB4 produced from arachidonic acid by 5-lipoxygenase is a potent chemoattractant of leukocytes. Leukotriene B4-12-hydroxydehydrogenase (LTB4-12-HD), which degrades LTB4, was downregulated in SHR rats compared with that in Wistar-Kyoto rats. Quantitative real-time PCR revealed that LTB4-12-HD was reduced by 63% and 58% in the NTS of adult SHR and prehypertensive SHR, respectively, compared with that in age-matched Wistar-Kyoto rats (n=6). 5-lipoxygenase gene expression was upregulated in the NTS of SHR (≈50%; n=6). LTB4 levels were increased in the NTS of the SHR, (17%; n=10, P",

keywords = "brain stem, hypertension, inflammation, sympathetic nervous system, CALCIUM IONOPHORE A23187, ANGIOTENSIN-II, NEUROGENIC HYPERTENSION, VASCULAR INFLAMMATION, ENDOTHELIAL-CELLS, ARACHIDONIC-ACID, BRAIN, RECEPTOR, ACTIVATION, LEUKOCYTES",

author = "Hidefumi Waki and Hendy, {Emma B} and Hindmarch, {Charlie C T} and Sabine Gouraud and Marie Toward and Sergey Kasparov and David Murphy and Paton, {Julian F R}",

year = "2013",

month = jan,

doi = "10.1161/HYPERTENSIONAHA.112.192252",

language = "English",

volume = "61",

pages = "194--201",

journal = "Hypertension",

issn = "1524-4563",

publisher = "Lippincott Williams and Wilkins",

number = "1",

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TY - JOUR

T1 - Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats

AU - Waki, Hidefumi

AU - Hendy, Emma B

AU - Hindmarch, Charlie C T

AU - Gouraud, Sabine

AU - Toward, Marie

AU - Kasparov, Sergey

AU - Murphy, David

AU - Paton, Julian F R

PY - 2013/1

Y1 - 2013/1

N2 - Inflammation within the brain stem microvasculature has been associated with chronic cardiovascular diseases. We found that the expression of several enzymes involved in arachidonic acid-leukotriene B4 (LTB4) production was altered in nucleus tractus solitarii (NTS) of spontaneously hypertensive rat (SHR). LTB4 produced from arachidonic acid by 5-lipoxygenase is a potent chemoattractant of leukocytes. Leukotriene B4-12-hydroxydehydrogenase (LTB4-12-HD), which degrades LTB4, was downregulated in SHR rats compared with that in Wistar-Kyoto rats. Quantitative real-time PCR revealed that LTB4-12-HD was reduced by 63% and 58% in the NTS of adult SHR and prehypertensive SHR, respectively, compared with that in age-matched Wistar-Kyoto rats (n=6). 5-lipoxygenase gene expression was upregulated in the NTS of SHR (≈50%; n=6). LTB4 levels were increased in the NTS of the SHR, (17%; n=10, P

AB - Inflammation within the brain stem microvasculature has been associated with chronic cardiovascular diseases. We found that the expression of several enzymes involved in arachidonic acid-leukotriene B4 (LTB4) production was altered in nucleus tractus solitarii (NTS) of spontaneously hypertensive rat (SHR). LTB4 produced from arachidonic acid by 5-lipoxygenase is a potent chemoattractant of leukocytes. Leukotriene B4-12-hydroxydehydrogenase (LTB4-12-HD), which degrades LTB4, was downregulated in SHR rats compared with that in Wistar-Kyoto rats. Quantitative real-time PCR revealed that LTB4-12-HD was reduced by 63% and 58% in the NTS of adult SHR and prehypertensive SHR, respectively, compared with that in age-matched Wistar-Kyoto rats (n=6). 5-lipoxygenase gene expression was upregulated in the NTS of SHR (≈50%; n=6). LTB4 levels were increased in the NTS of the SHR, (17%; n=10, P

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KW - inflammation

KW - sympathetic nervous system

KW - CALCIUM IONOPHORE A23187

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KW - NEUROGENIC HYPERTENSION

KW - VASCULAR INFLAMMATION

KW - ENDOTHELIAL-CELLS

KW - ARACHIDONIC-ACID

KW - BRAIN

KW - RECEPTOR

KW - ACTIVATION

KW - LEUKOCYTES

U2 - 10.1161/HYPERTENSIONAHA.112.192252

DO - 10.1161/HYPERTENSIONAHA.112.192252

M3 - Article (Academic Journal)

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SP - 194

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JO - Hypertension

JF - Hypertension

IS - 1

ER -

Excessive leukotriene B4 in nucleus tractus solitarii is prohypertensive in spontaneously hypertensive rats

Abstract

Keywords

UN SDGs

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Projects

Bilateral BBSRC-FAPESP: Behavioural and neuroendocrine mechanisms regulating hydromineral homeostasis - a lifelong perspective

USA - Novel insights into the mechanisms of salt appetite

Bilateral BBSRC-FAPESP: Amelioration of the autonomic imbalances of old age with exercise exploring the molecular and physiological mechanisms

Cite this