Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

Robert Spaull; Bryony McPherson; Andriana Gialeli; Aled Clayton; James Uney; Axel Heep; Óscar Cordero-Llana

doi:10.1016/j.ijdevneu.2019.01.004

Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

Robert Spaull, Bryony McPherson, Andriana Gialeli, Aled Clayton, James Uney, Axel Heep^*, Óscar Cordero-Llana

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

25 Citations (Scopus)

119 Downloads (Pure)

Abstract

Background
Preterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs.
Methods
This study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.
Results
NTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.
Conclusion
This is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.

Original language	English
Pages (from-to)	59-65
Number of pages	7
Journal	International Journal of Developmental Neuroscience
Volume	73
Early online date	9 Jan 2019
DOIs	https://doi.org/10.1016/j.ijdevneu.2019.01.004
Publication status	Published - Apr 2019

Keywords

Cerebral palsy
Cerebrospinal fluid
Exosome
Intraventricular hemorrhage
Micro RNA
Preterm infant

Access to Document

10.1016/j.ijdevneu.2019.01.004

Full-text PDF (accepted author manuscript)
This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://www.sciencedirect.com/science/article/pii/S0736574818303058 . Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 1.06 MBLicence: CC BY-NC-ND

Handle.net

Persistent link

Cite this

@article{b274fc7f4154494480c7c89b19106501,

title = "Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus",

abstract = "BackgroundPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs. MethodsThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.ResultsNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.ConclusionThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus. ",

keywords = "Cerebral palsy, Cerebrospinal fluid, Exosome, Intraventricular hemorrhage, Micro RNA, Preterm infant",

author = "Robert Spaull and Bryony McPherson and Andriana Gialeli and Aled Clayton and James Uney and Axel Heep and {\'O}scar Cordero-Llana",

year = "2019",

month = apr,

doi = "10.1016/j.ijdevneu.2019.01.004",

language = "English",

volume = "73",

pages = "59--65",

journal = "International Journal of Developmental Neuroscience",

issn = "0736-5748",

publisher = "Elsevier",

}

TY - JOUR

T1 - Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

AU - Spaull, Robert

AU - McPherson, Bryony

AU - Gialeli, Andriana

AU - Clayton, Aled

AU - Uney, James

AU - Heep, Axel

AU - Cordero-Llana, Óscar

PY - 2019/4

Y1 - 2019/4

N2 - BackgroundPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs. MethodsThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.ResultsNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.ConclusionThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.

AB - BackgroundPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs. MethodsThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.ResultsNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.ConclusionThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.

KW - Cerebral palsy

KW - Cerebrospinal fluid

KW - Exosome

KW - Intraventricular hemorrhage

KW - Micro RNA

KW - Preterm infant

UR - http://www.scopus.com/inward/record.url?scp=85059801741&partnerID=8YFLogxK

U2 - 10.1016/j.ijdevneu.2019.01.004

DO - 10.1016/j.ijdevneu.2019.01.004

M3 - Article (Academic Journal)

C2 - 30639393

AN - SCOPUS:85059801741

VL - 73

SP - 59

EP - 65

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

SN - 0736-5748

ER -

Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Characteristics of exosomes in the cerebrospinal fluid of preterm infants

Nanomolecular signalling in the developing brain: Characteristics of exosomes in the cerebrospinal fluid of preterm infants

Elizabeth Blackwell Institute Clinical Primer

Dr Oscar Cordero Llana

Cite this

Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Research output

Characteristics of exosomes in the cerebrospinal fluid of preterm infants

Nanomolecular signalling in the developing brain: Characteristics of exosomes in the cerebrospinal fluid of preterm infants

Prizes

Elizabeth Blackwell Institute Clinical Primer

Profiles

Dr Oscar Cordero Llana

Cite this