TY - JOUR
T1 - Exosomes populate the cerebrospinal fluid of preterm infants with post-haemorrhagic hydrocephalus
AU - Spaull, Robert
AU - McPherson, Bryony
AU - Gialeli, Andriana
AU - Clayton, Aled
AU - Uney, James
AU - Heep, Axel
AU - Cordero-Llana, Óscar
PY - 2019/4
Y1 - 2019/4
N2 - BackgroundPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs. MethodsThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.ResultsNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.ConclusionThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.
AB - BackgroundPreterm infants are at risk of germinal matrix haemorrhage-intraventricular haemorrhage (GMH-IVH) which leads to post-haemorrhagic hydrocephalus (PHH) in 30% of infants; this is associated with moderate-severe neurodevelopmental impairment and confers significant risk of cerebral palsy. There are however no predictive indicators of the severity or long-term outcome after GMH-IVH. In recent years, endosome-derived extracellular vesicles (EVs) or exosomes have been isolated from biofluids and shown to mediate intercellular communication via selective enrichment in proteins and micro-RNAs. MethodsThis study aimed to isolate and characterise EVs from the cerebrospinal fluid (CSF) of 3 preterm infants with PHH using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) with immunogold protein labelling, and micro-RNA analysis.ResultsNTA of unaltered CSF revealed a heterogeneous and dynamic population of EVs. Exosomal-sized EVs were isolated by differential ultracentrifugation and TEM confirmed the presence of CD63+ and CD81+ exosomes. The micro-RNAs miR-9, miR-17, miR-26a, miR-124 and miR-1911 were detected within the exosome-enriched fraction and profiled over time.ConclusionThis is the first reported characterisation of exosomes from the CSF of preterm infants with post-haemorrhagic hydrocephalus.
KW - Cerebral palsy
KW - Cerebrospinal fluid
KW - Exosome
KW - Intraventricular hemorrhage
KW - Micro RNA
KW - Preterm infant
UR - http://www.scopus.com/inward/record.url?scp=85059801741&partnerID=8YFLogxK
U2 - 10.1016/j.ijdevneu.2019.01.004
DO - 10.1016/j.ijdevneu.2019.01.004
M3 - Article (Academic Journal)
C2 - 30639393
AN - SCOPUS:85059801741
SN - 0736-5748
VL - 73
SP - 59
EP - 65
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
ER -