Abstract
CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue-engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl-O-methyl-cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time-dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.
Original language | English |
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Pages (from-to) | 473-475 |
Number of pages | 3 |
Journal | Experimental Dermatology |
Volume | 27 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2018 |
Bibliographical note
© 2017 The Authors. Experimental Dermatology Published by John Wiley & Sons Ltd.Keywords
- Cytochrome P-450 CYP3A/metabolism
- Humans
- Liver/enzymology
- Models, Biological
- Skin/enzymology
- Tissue Engineering