Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue-engineered skin equivalents

Sarah A Smith, Helen E Colley, Parveen Sharma, Klaudia M Slowik, Rowena Sison-Young, Andrew Sneddon, Steve Webb, Craig Murdoch

Research output: Contribution to journalArticle (Academic Journal)

Abstract

CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue-engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl-O-methyl-cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time-dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.

Original languageEnglish
Pages (from-to)473-475
Number of pages3
JournalExperimental Dermatology
Volume27
Issue number5
DOIs
Publication statusPublished - May 2018

Bibliographical note

© 2017 The Authors. Experimental Dermatology Published by John Wiley & Sons Ltd.

Keywords

  • Cytochrome P-450 CYP3A/metabolism
  • Humans
  • Liver/enzymology
  • Models, Biological
  • Skin/enzymology
  • Tissue Engineering

Fingerprint Dive into the research topics of 'Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue-engineered skin equivalents'. Together they form a unique fingerprint.

Cite this