Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa, Hilary A. Overton, John P. Moore, Anthony J. Wilkinson, R. Leo Brady, Sally J. Lewis, Ian M. Jones*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

47 Citations (Scopus)

Abstract

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

Original languageEnglish
Pages (from-to)765-773
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Jan 1990

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