Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa; Hilary A. Overton; John P Moore; Anthony J. Wilkinson; R. Leo Brady; Sally J. Lewis; Ian M Jones

doi:10.1089/aid.1990.6.765

Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa, Hilary A. Overton, John P Moore, Anthony J. Wilkinson, R. Leo Brady, Sally J. Lewis, Ian M Jones^*

^*Corresponding author for this work

School of Biochemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

59 Citations (Scopus)

Abstract

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 10⁹ cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

Original language	English
Pages (from-to)	765-773
Number of pages	9
Journal	AIDS Research and Human Retroviruses
Volume	6
Issue number	6
DOIs	https://doi.org/10.1089/aid.1990.6.765
Publication status	Published - 1 Jan 1990

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title = "Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro",

abstract = "The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.",

author = "Yuko Morikawa and Overton, {Hilary A.} and Moore, {John P} and Wilkinson, {Anthony J.} and Brady, {R. Leo} and Lewis, {Sally J.} and Jones, {Ian M}",

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AU - Morikawa, Yuko

AU - Overton, Hilary A.

AU - Moore, John P

AU - Wilkinson, Anthony J.

AU - Brady, R. Leo

AU - Lewis, Sally J.

AU - Jones, Ian M

PY - 1990/1/1

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AB - The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

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JF - AIDS Research and Human Retroviruses

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ER -

Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Abstract

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