Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa, Hilary A. Overton, John P Moore, Anthony J. Wilkinson, R. Leo Brady, Sally J. Lewis, Ian M Jones*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

59 Citations (Scopus)

Abstract

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

Original languageEnglish
Pages (from-to)765-773
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Jan 1990

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