Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa; Hilary A. Overton; John P Moore; Anthony J. Wilkinson; R. Leo Brady; Sally J. Lewis; Ian M Jones

doi:10.1089/aid.1990.6.765

Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Yuko Morikawa
, Hilary A. Overton
, John P Moore
, Anthony J. Wilkinson
, R. Leo Brady
, Sally J. Lewis
, Ian M Jones^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

59 Citations (Scopus)

Abstract

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 10⁹ cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

Original language	English
Pages (from-to)	765-773
Number of pages	9
Journal	AIDS Research and Human Retroviruses
Volume	6
Issue number	6
DOIs	https://doi.org/10.1089/aid.1990.6.765
Publication status	Published - 1 Jan 1990

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title = "Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro",

abstract = "The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.",

author = "Yuko Morikawa and Overton, \{Hilary A.\} and Moore, \{John P\} and Wilkinson, \{Anthony J.\} and Brady, \{R. Leo\} and Lewis, \{Sally J.\} and Jones, \{Ian M\}",

year = "1990",

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doi = "10.1089/aid.1990.6.765",

language = "English",

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}

TY - JOUR

T1 - Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

AU - Morikawa, Yuko

AU - Overton, Hilary A.

AU - Moore, John P

AU - Wilkinson, Anthony J.

AU - Brady, R. Leo

AU - Lewis, Sally J.

AU - Jones, Ian M

PY - 1990/1/1

Y1 - 1990/1/1

N2 - The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

AB - The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

UR - https://www.scopus.com/pages/publications/0025132616

U2 - 10.1089/aid.1990.6.765

DO - 10.1089/aid.1990.6.765

M3 - Article (Academic Journal)

C2 - 2364018

AN - SCOPUS:0025132616

SN - 0889-2229

VL - 6

SP - 765

EP - 773

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - 6

ER -

Expression of HIV-1 gp120 and Human Soluble CD4 by Recombinant Baculoviruses and their Interaction In Vitro

Abstract

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