Expression of laccase gene lcc1 in Coprinopsis cinerea under control of various basidiomycetous promoters

S Kilaru, PJ Hoegger, A Majcherczyk, AC Burns, K Shishido, A Bailey, GD Foster, U Kües

Research output: Contribution to journalArticle (Academic Journal)peer-review

55 Citations (Scopus)

Abstract

Coprinopsis cinerea laccase gene lcc1 was expressed in this basidiomycete under naturally non-inductive conditions using various homologous and heterologous promoters. Laccase expression was achieved in solid and liquid media with promoter sequences from the C. cinerea tub1 gene, the Agaricus bisporus gpdII gene, the Lentinus edodes priA gene and the Schizophyllum commune Sc3 gene. As measured by enzyme activity in liquid cultures, a 277-bp gpdII promoter fragment, followed by a 423-bp priA fragment, was most efficient. A shorter priA sequence of 372 bp was inactive. tub1 promoter fragments were reasonably active, whereas the S. commune Sc3 promoter sequence was less active, in comparison. Irrespective of the promoter used, addition of copper to the medium increased enzymatic activities for highly active transformants by 10- to 50-fold and for less active transformants for 2- to 7-fold. The highest enzymatic activities (3 U/ml) were reached with the gpdII promoter in the presence of 0.1 mM CuSO4.
Translated title of the contributionExpression of laccase gene lcc1 in Coprinopsis cinerea under control of various basidiomycetous promoters
Original languageEnglish
Pages (from-to)200 - 210
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume71 (2)
DOIs
Publication statusPublished - Jun 2006

Bibliographical note

Publisher: Springer

Fingerprint

Dive into the research topics of 'Expression of laccase gene <i>lcc1</i> in <i>Coprinopsis cinerea</i> under control of various basidiomycetous promoters'. Together they form a unique fingerprint.

Cite this