Fabrication of functional bioinorganic nanoconstructs by polymer-silica wrapping of individual myoglobin molecules

Biru Hu, Mei Li, Sajanikumari Sadasivan, Avinash J. Patil, Stephen Mann*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

5 Citations (Scopus)

Abstract

Discrete core-shell hybrid nanoparticles comprising individual met-myoglobin (met-Mb) molecules incarcerated within an ultrathin polymer/silica shell were prepared without loss of biofunctionality by a facile self-assembly procedure. Solubilisation of met-Mb in cyclohexane in the near-absence of water was achieved by wrapping individual protein molecules in the amphiphilic triblock copolymer poly(ethylene-oxide)(19)-poly(propylene-oxide)(69)-poly(ethylene-oxide)(19) (EO19-PO69-EO19, P123). Addition of tetramethoxysilane to the met-Mb/P123 conjugates in cyclohexane produced discrete nanoparticles that contained protein, polymer and silica, and which were 3-5.5 nm in size, consistent with the entrapment of single molecules of met-Mb. The hybrid nanoconstructs were isolated and re-dispersed in water without loss of secondary structure, and remained functionally active with respect to redox reactions and CO and O-2 ligand binding at the porphyrin metallocentre. The incarcerated met-Mb biomolecules showed enhanced thermal stability up to a temperature of around 85 degrees C. These properties, along with the high biocompatibility of silica and P123, suggest that the silicified protein-polymer constructs could be utilised as functional nanoscale components in bionanotechnology.

Original languageEnglish
Pages (from-to)1031-1036
Number of pages6
JournalNanoscale
Volume3
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • ORGANOCLAY BUILDING-BLOCKS
  • SOL-GEL MATERIALS
  • ZIRCONIUM-PHOSPHATE
  • ENZYMATIC-ACTIVITY
  • ENCAPSULATION
  • PROTEINS
  • IMMOBILIZATION
  • NANOCOMPOSITES
  • INTERCALATION
  • ADSORPTION

Cite this