Fabrication of Micropatterned Dipeptide Hydrogels by Acoustic Trapping of Stimulus-Responsive Coacervate Droplets

Madeleine K. Nichols, Ravinash Krishna Kumar, Philip G. Bassindale, Liangfei Tian, Adrian C. Barnes, Bruce W. Drinkwater, Avinash J. Patil*, Stephen Mann

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

22 Citations (Scopus)
260 Downloads (Pure)

Abstract

Acoustic standing waves offer an excellent opportunity to trap and spatially manipulate colloidal objects. This noncontact technique is used for the in situ formation and patterning in aqueous solution of 1D or 2D arrays of pH-responsive coacervate microdroplets comprising poly(diallyldimethylammonium) chloride and the dipeptide N-fluorenyl-9-methoxy-carbonyl-D-alanine-D-alanine. Decreasing the pH of the preformed droplet arrays results in dipeptide nanofilament self-assembly and subsequent formation of a micropatterned supramolecular hydrogel that can be removed as a self-supporting monolith. Guest molecules such as molecular dyes, proteins, and oligonucleotides are sequestered specifically within the coacervate droplets during acoustic processing to produce micropatterned hydrogels containing spatially organized functional components. Using this strategy, the site-specific isolation of multiple enzymes to drive a catalytic cascade within the micropatterned hydrogel films is exploited.

Original languageEnglish
Article number1800739
Number of pages10
JournalSmall
Volume14
Issue number26
Early online date3 May 2018
DOIs
Publication statusPublished - 27 Jun 2018

Structured keywords

  • Bristol BioDesign Institute
  • BrisSynBio

Keywords

  • acoustic trapping
  • coacervates
  • hydrogels
  • micropatterning
  • self-assembly

Fingerprint

Dive into the research topics of 'Fabrication of Micropatterned Dipeptide Hydrogels by Acoustic Trapping of Stimulus-Responsive Coacervate Droplets'. Together they form a unique fingerprint.

Cite this