Abstract
FANCM suppresses crossovers in plants by unwinding recombination intermediates. In wheat, crossovers are skewed toward the chromosome ends, thus limiting generation of novel allelic combinations. Here, we observe that FANCM maintains the obligate crossover in tetraploid and hexaploid wheat, thus ensuring that every chromosome pair exhibits at least one crossover, by localizing class I crossover protein HEI10 at pachytene. FANCM also suppresses class II crossovers that increased 2.6-fold in fancm msh5 quadruple mutants. These data are consistent with a role for FANCM in second-end capture of class I designated crossover sites, whilst FANCM is also required to promote formation of non-crossovers. In hexaploid wheat, genetic mapping reveals that crossovers increase by 31% in fancm compared to wild type, indicating that fancm could be an effective tool to accelerate breeding. Crossover rate differences in fancm correlate with wild type crossover distributions, suggesting that chromatin may influence the recombination landscape in similar ways in both wild type and fancm.
| Original language | English |
|---|---|
| Article number | 3644 |
| Journal | Nature Communications |
| Volume | 13 |
| Issue number | 1 |
| Early online date | 25 Jun 2022 |
| DOIs | |
| Publication status | Published - Dec 2022 |
Bibliographical note
Funding Information:We would like to thank Neelam Dave for technical support, Helen Harper as sLola project coordinator, Claire Meade for support with crosses, and the sLola Steering Committee for helpful advice throughout the project. We thank Tobin Florio at flozbox-science for illustrating Fig. . This work was funded by UKRI through a BBSRC strategic Long and Large grant (sLoLa) BB/N002628/1.
Publisher Copyright:
© 2022, The Author(s).