Fast identification and quantification of uropatho-genic E. coli through cluster analysis

Jean-Baptiste Vendeville, Mathew John Kyriakides, Yuiko Takebayashi, Josephine Ndoa Dorh*, James Spencer*, M C Galan*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Rapid diagnostic tools to detect, identify and enumerate bacteria are key to
maintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broadspectrum agents. In this study, a 15-min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC
12241) and Uropathogenic Escherichia coli (UPEC) strains (NCTC 9001, NCTC 13958, J96, and CTF073) at clinically relevant concentrations in tryptic soy broth (TSB) (103 -108 CFU/ml) and in urine (105 -108 CFU/ml). This fast, simple and efficient assay offers a step forward towards efficient point-of-care diagnostics for common Urinary Tract Infections (UTI).
Original languageEnglish
Article number 242–252
JournalACS Biomaterials Science and Engineering
Volume8
Issue number1
Early online date13 Dec 2021
DOIs
Publication statusE-pub ahead of print - 13 Dec 2021

Keywords

  • Mannose
  • Escherichia coli
  • Agglutination
  • UPEC
  • diagnostics

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