Fast identification and quantification of uropatho-genic E. coli through cluster analysis

Jean-Baptiste Vendeville; Mathew John Kyriakides; Yuiko Takebayashi; Josephine Ndoa Dorh; James Spencer; M C Galan

doi:10.1021/acsbiomaterials.1c00732

Fast identification and quantification of uropatho-genic E. coli through cluster analysis

Jean-Baptiste Vendeville, Mathew John Kyriakides, Yuiko Takebayashi, Josephine Ndoa Dorh^*, James Spencer^*, M C Galan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Rapid diagnostic tools to detect, identify and enumerate bacteria are key to
maintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broadspectrum agents. In this study, a 15-min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC
12241) and Uropathogenic Escherichia coli (UPEC) strains (NCTC 9001, NCTC 13958, J96, and CTF073) at clinically relevant concentrations in tryptic soy broth (TSB) (103 -108 CFU/ml) and in urine (105 -108 CFU/ml). This fast, simple and efficient assay offers a step forward towards efficient point-of-care diagnostics for common Urinary Tract Infections (UTI).

Original language	English
Article number	242–252
Journal	ACS Biomaterials Science and Engineering
Volume	8
Issue number	1
Early online date	13 Dec 2021
DOIs	https://doi.org/10.1021/acsbiomaterials.1c00732
Publication status	E-pub ahead of print - 13 Dec 2021

Keywords

Mannose
Escherichia coli
Agglutination
UPEC
diagnostics

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10.1021/acsbiomaterials.1c00732

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This is the accepted author manuscript (AAM). The final published version (version of record) is available online via American Chemical Society at https://doi.org/10.1021/acsbiomaterials.1c00732/. Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 2.24 MB

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title = "Fast identification and quantification of uropatho-genic E. coli through cluster analysis",

abstract = "Rapid diagnostic tools to detect, identify and enumerate bacteria are key tomaintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broadspectrum agents. In this study, a 15-min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC12241) and Uropathogenic Escherichia coli (UPEC) strains (NCTC 9001, NCTC 13958, J96, and CTF073) at clinically relevant concentrations in tryptic soy broth (TSB) (103 -108 CFU/ml) and in urine (105 -108 CFU/ml). This fast, simple and efficient assay offers a step forward towards efficient point-of-care diagnostics for common Urinary Tract Infections (UTI).",

keywords = "Mannose, Escherichia coli, Agglutination, UPEC, diagnostics",

author = "Jean-Baptiste Vendeville and Kyriakides, {Mathew John} and Yuiko Takebayashi and Dorh, {Josephine Ndoa} and James Spencer and Galan, {M C}",

year = "2021",

month = dec,

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doi = "10.1021/acsbiomaterials.1c00732",

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TY - JOUR

T1 - Fast identification and quantification of uropatho-genic E. coli through cluster analysis

AU - Vendeville, Jean-Baptiste

AU - Kyriakides, Mathew John

AU - Takebayashi, Yuiko

AU - Dorh, Josephine Ndoa

AU - Spencer, James

AU - Galan, M C

PY - 2021/12/13

Y1 - 2021/12/13

N2 - Rapid diagnostic tools to detect, identify and enumerate bacteria are key tomaintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broadspectrum agents. In this study, a 15-min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC12241) and Uropathogenic Escherichia coli (UPEC) strains (NCTC 9001, NCTC 13958, J96, and CTF073) at clinically relevant concentrations in tryptic soy broth (TSB) (103 -108 CFU/ml) and in urine (105 -108 CFU/ml). This fast, simple and efficient assay offers a step forward towards efficient point-of-care diagnostics for common Urinary Tract Infections (UTI).

AB - Rapid diagnostic tools to detect, identify and enumerate bacteria are key tomaintaining effective antibiotic stewardship and avoiding the unnecessary prescription of broadspectrum agents. In this study, a 15-min agglutination assay is developed that relies on the use of mannose-functionalized polymeric microspheres in combination with cluster analysis. This allows for the identification and enumeration of laboratory (BW25113), clinical isolate (NCTC12241) and Uropathogenic Escherichia coli (UPEC) strains (NCTC 9001, NCTC 13958, J96, and CTF073) at clinically relevant concentrations in tryptic soy broth (TSB) (103 -108 CFU/ml) and in urine (105 -108 CFU/ml). This fast, simple and efficient assay offers a step forward towards efficient point-of-care diagnostics for common Urinary Tract Infections (UTI).

KW - Mannose

KW - Escherichia coli

KW - Agglutination

KW - UPEC

KW - diagnostics

U2 - 10.1021/acsbiomaterials.1c00732

DO - 10.1021/acsbiomaterials.1c00732

M3 - Article (Academic Journal)

C2 - 34894660

SN - 2373-9878

VL - 8

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

IS - 1

M1 - 242–252

ER -

Fast identification and quantification of uropatho-genic E. coli through cluster analysis

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Keywords

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