FGF but not EGF induces phosphorylation of the cAMP response element binding protein in olfactory mucosa-derived cell cultures

P Barraud, X He, C Zhao, MA Caldwell, RJ Franklin

Research output: Contribution to journalArticle (Academic Journal)

3 Citations (Scopus)

Abstract

The stem/progenitor cells of the olfactory epithelium are potentially useful cells for autologous cell-based therapy because of their relative accessibility compared to other sources of neural stem cells. However, they have very limited potential to self-renew in vitro under growth factor stimulation compared to central nervous system-derived stem/progenitor cells. Using a sphere-forming assay and immunocytochemistry to identify cells that contained phosphorylated cAMP response element binding protein (pCREB) as an indicator of cell responsiveness to growth factor activation, we found that olfactory-spheres primed with FGF2 responded to FGF2 and EGF stimulation. In contrast, olfactory-spheres primed with EGF failed to respond to FGF2 or EGF stimulation despite the detection of FGFR1 and EGFR and their transcripts. These data demonstrate that FGF2 but not EGF permit the maintenance of a subset of cells responsive to FGF2 and EGF, whereas EGF induces unresponsive to either growth factor possibly via intrinsic mechanisms of regulation.
Translated title of the contributionFGF but not EGF induces phosphorylation of the cAMP response element binding protein in olfactory mucosa-derived cell cultures
Original languageEnglish
Pages (from-to)1489 - 1499
Number of pages11
JournalExperimental Cell Research
Volume15;316(9)
DOIs
Publication statusPublished - May 2010

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