Abstract
INTRODUCTION/AIMS: Raman spectroscopy is an emerging technique for the evaluation of muscle disease. In this study we evaluate the ability of in vivo intramuscular Raman spectroscopy to detect the effects of voluntary running in the mdx model of Duchenne muscular dystrophy (DMD). We also compare mdx data with muscle spectra from human DMD patients.
METHODS: Thirty 90-day-old mdx mice were randomly allocated to an exercised group (48-hour access to a running wheel) and an unexercised group (n = 15 per group). In vivo Raman spectra were collected from both gastrocnemius muscles and histopathological assessment subsequently performed. Raman data were analyzed using principal component analysis-fed linear discriminant analysis (PCA-LDA). Exercised and unexercised mdx muscle spectra were compared with human DMD samples using cosine similarity.
RESULTS: Exercised mice ran an average of 6.5 km over 48 hours, which induced a significant increase in muscle necrosis (P = .03). PCA-LDA scores were significantly different between the exercised and unexercised groups (P < .0001) and correlated significantly with distance run (P = .01). Raman spectra from exercised mice more closely resembled human spectra than those from unexercised mice.
DISCUSSION: Raman spectroscopy provides a readout of the biochemical alterations in muscle in both the mdx mouse and human DMD muscle.
| Original language | English |
|---|---|
| Pages (from-to) | 362-369 |
| Number of pages | 8 |
| Journal | Muscle and Nerve |
| Volume | 66 |
| Issue number | 3 |
| Early online date | 28 Jun 2022 |
| DOIs | |
| Publication status | Published - 19 Aug 2022 |
Bibliographical note
Funding Information:The authors thank the Oxford Brain Bank (supported by the Medical Research Council), Brains for Dementia Research (Alzheimer Society and Alzheimer Research UK), Autistica UK, and the NIHR Oxford Biomedical Research Centre. We also thank the patients for donating their muscle for medical research. Finally, we thank the staff of the Biological Services Unit, University of Sheffield, for their dedicated support.
Funding Information:
Academy of Medical Sciences (starter grant to J.J.P.A.); Medical Research Council (Confidence in Concept Awards to J.J.P.A., J.C.D., R.J.M., P.J.S.); National Institute for Health Research (NIHR) (Senior Investigator Award to P.J.S.); NIHR Sheffield Biomedical Research Centre (to P.J.S.).
Funding Information:
The authors thank the Oxford Brain Bank (supported by the Medical Research Council), Brains for Dementia Research (Alzheimer Society and Alzheimer Research UK), Autistica UK, and the NIHR Oxford Biomedical Research Centre. We also thank the patients for donating their muscle for medical research. Finally, we thank the staff of the Biological Services Unit, University of Sheffield, for their dedicated support.
Publisher Copyright:
© 2022 The Authors. Muscle & Nerve published by Wiley Periodicals LLC.
Access to Document
- Full-text PDF (final published version)
This is the final published version of the article (version of record). It first appeared online via Wiley at https://doi.org/10.1002/mus.27671 . Please refer to any applicable terms of use of the publisher.