Flexible approach to automated protein crystallization

T. J. Oldfield; T. A. Ceska; R. L. Brady

doi:10.1107/S0021889891001632

Flexible approach to automated protein crystallization

T. J. Oldfield^*
, T. A. Ceska
, R. L. Brady

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

16 Citations (Scopus)

Abstract

An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	Journal of Applied Crystallography
Volume	24
Issue number	pt 3
DOIs	https://doi.org/10.1107/S0021889891001632
Publication status	Published - 1 Jun 1991

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title = "Flexible approach to automated protein crystallization",

abstract = "An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.",

author = "Oldfield, \{T. J.\} and Ceska, \{T. A.\} and Brady, \{R. L.\}",

year = "1991",

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language = "English",

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T1 - Flexible approach to automated protein crystallization

AU - Oldfield, T. J.

AU - Ceska, T. A.

AU - Brady, R. L.

PY - 1991/6/1

Y1 - 1991/6/1

N2 - An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.

AB - An automated system for protein crystallization trials is described. Similar to commercial systems in operation, this system is readily assembled at a far lower cost. The software runs on either a microcomputer or a VAX and is both versatile and readily modified for special requirements. The system is capable of pipetting polyethylene glycol solutions accurately. Root-mean-square deviations in the accuracy of pipetted well solutions were similar to results obtained manually. Factors influencing reproducibility are discussed.

UR - https://www.scopus.com/pages/publications/0026172472

U2 - 10.1107/S0021889891001632

DO - 10.1107/S0021889891001632

M3 - Article (Academic Journal)

AN - SCOPUS:0026172472

SN - 0021-8898

VL - 24

SP - 255

EP - 260

JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - pt 3

ER -

Flexible approach to automated protein crystallization

Abstract

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