Fluorescent protein-based technologies: shedding new light on the plant endomembrane system

Imogen Sparkes, Federica Brandizzi

Research output: Contribution to journalReview article (Academic Journal)peer-review

16 Citations (Scopus)

Abstract

Without doubt, GFP and spectral derivatives have revolutionized the way biologists approach their journey toward the discovery of how plant cells function. It is fascinating that in its early days GFP was used merely for localization studies, but as time progressed researchers successfully explored new avenues to push the power of GFP technology to reach new and exciting research frontiers. This has had a profound impact on the way we can now study complex and dynamic systems such as plant endomembranes. Here we briefly describe some of the approaches where GFP has revolutionized in vivo studies of protein distribution and dynamics and focus on two emerging approaches for the application of GFP technology in plant endomembranes, namely optical tweezers and forward genetics approaches, which are based either on the light or on genetic manipulation of secretory organelles to gain insights on the factors that control their activities and integrity.

Original languageEnglish
Pages (from-to)96-107
Number of pages12
JournalPlant Journal
Volume70
Issue number1
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Cell Membrane
  • Endocytosis
  • Green Fluorescent Proteins
  • Optical Tweezers
  • Organelles
  • Plant Cells
  • Plants
  • Protein Interaction Mapping
  • Journal Article
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

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