Biochemistry identified a mild hyperproteinaemia. Blood extraction and PCR detected Leishmania species, Hepatozoon species and “Candidatus Mycoplasma haemominutum” (CMhm) DNA. Subsequent sequencing identified Hepatozoon felis. Additionally, the rRNA internal transcribed spacer 1 (ITS1) locus of Leishmania infantum was partially sequenced and phylogeny showed it to cluster with species derived from dogs in Italy and Uzbekistan, and a human being in France.
Allopurinol treatment was administered for six months. Clinical signs resolved in the second month of treatment with no deterioration eight months post-treatment cessation.
Quantitative PCR and ELISA were used to monitor L infantum blood DNA and antibody levels. The cat had high L infantum DNA levels pre-treatment that gradually declined during treatment but increased eight months post-treatment cessation. Similarly, ELISA revealed high levels of antibodies pre-treatment which gradually declined during treatment and increased slightly eight months post-treatment cessation. The cat remained PCR positive for CMhm and Hepatozoon species throughout the study. There was no clinical evidence of relapse twenty-four months post-treatment.
Relevance and novel information: To our knowledge this is the first clinical report of a cat with leishmaniosis with H felis and CMhm co-infections. The high L infantum DNA levels post-treatment cessation might indicate that although the lesions had resolved, prolonged or an alternative treatment could have been considered.
|Number of pages||6|
|Journal||Journal of Feline Medicine and Surgery Open Reports|
|Early online date||14 Nov 2017|
|Publication status||Published - Dec 2017|