Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

Yenenesh K Tekletsion; Hannah Christensen; Adam Finn

doi:10.1016/j.mimet.2018.02.003

Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

Yenenesh K Tekletsion^*, Hannah Christensen, Adam Finn

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

2 Citations (Scopus)

307 Downloads (Pure)

Abstract

Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R² = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.

Original language	English
Pages (from-to)	100-103
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	146
Early online date	7 Feb 2018
DOIs	https://doi.org/10.1016/j.mimet.2018.02.003
Publication status	Published - Mar 2018

Keywords

Transcriptomics
NanoString
Gene expression
Neisseria meningitidis
mRNA

Access to Document

10.1016/j.mimet.2018.02.003

Full-text PDF (accepted author manuscript)
This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://www.sciencedirect.com/science/article/pii/S0167701218300903 . Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 862 KBLicence: CC BY-NC-ND

Handle.net

Persistent link

Cite this

@article{39575e587a0b4cefaed3bd9a395c5d92,

title = "Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform",

abstract = "Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R2 = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.",

keywords = "Transcriptomics, NanoString, Gene expression, Neisseria meningitidis, mRNA",

author = "Tekletsion, {Yenenesh K} and Hannah Christensen and Adam Finn",

note = "Copyright {\textcopyright} 2017. Published by Elsevier B.V.",

year = "2018",

month = mar,

doi = "10.1016/j.mimet.2018.02.003",

language = "English",

volume = "146",

pages = "100--103",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

AU - Tekletsion, Yenenesh K

AU - Christensen, Hannah

AU - Finn, Adam

PY - 2018/3

Y1 - 2018/3

N2 - Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R2 = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.

AB - Detection of bacterial gene transcripts in low density mucosal samples is challenging. We evaluated the NanoString nCounter system for transcript detection in Neisseria meningitidis (Nm) cultures. The method was sensitive, reproducible (R2 = 0.99) and demonstrated changes in gene expression. Studying Nm transcripts from pharyngeal samples may be feasible using this approach.

KW - Transcriptomics

KW - NanoString

KW - Gene expression

KW - Neisseria meningitidis

KW - mRNA

UR - http://www.scopus.com/inward/record.url?scp=85042178518&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2018.02.003

DO - 10.1016/j.mimet.2018.02.003

M3 - Article (Academic Journal)

C2 - 29425856

VL - 146

SP - 100

EP - 103

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

ER -

Gene detection and expression profiling of Neisseria meningitidis using NanoString nCounter platform

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this