Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering

Lakshmi S Vijayachandran, Deepak B Thimiri Govinda Raj, Evelina Edelweiss, Kapil Gupta, Josef Maier, Valentin Gordeliy, Daniel J Fitzgerald, Imre Berger

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Most essential activities in eukaryotic cells are catalyzed by large multiprotein assemblies containing up to ten or more interlocking subunits. The vast majority of these protein complexes are not easily accessible for high resolution studies aimed at unlocking their mechanisms, due to their low cellular abundance and high heterogeneity. Recombinant overproduction can resolve this bottleneck and baculovirus expression vector systems (BEVS) have emerged as particularly powerful tools for the provision of eukaryotic multiprotein complexes in high quality and quantity. Recently, synthetic biology approaches have begun to make their mark in improving existing BEVS reagents by de novo design of streamlined transfer plasmids and by engineering the baculovirus genome. Here we present OmniBac, comprising new custom designed reagents that further facilitate the integration of heterologous genes into the baculovirus genome for multiprotein expression. Based on comparative genome analysis and data mining, we herein present a blueprint to custom design and engineer the entire baculovirus genome for optimized production properties using a bottom-up synthetic biology approach.

Original languageEnglish
Pages (from-to)279-87
Number of pages9
JournalBioengineered Online
Volume4
Issue number5
DOIs
Publication statusPublished - 19 Jan 2013

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • Baculoviridae
  • Chromosome Mapping
  • Cloning, Molecular
  • Data Mining
  • Eukaryotic Cells
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Engineering
  • Genetic Vectors
  • Genome, Viral
  • Multiprotein Complexes
  • Plasmids
  • Synthetic Biology

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