Gene-Mediated Chemical Communication in Synthetic Protocell Communities

T. Y.Dora Tang; Dario Cecchi; Giorgio Fracasso; Davide Accardi; Angelique Coutable-Pennarun; Sheref S. Mansy; Adam W. Perriman; J. L.Ross Anderson; Stephen Mann

doi:10.1021/acssynbio.7b00306

Gene-Mediated Chemical Communication in Synthetic Protocell Communities

T. Y.Dora Tang^*, Dario Cecchi, Giorgio Fracasso, Davide Accardi, Angelique Coutable-Pennarun, Sheref S. Mansy, Adam W. Perriman, J. L.Ross Anderson, Stephen Mann

^*Corresponding author for this work

Research output: Contribution to journal › Letter (Academic Journal) › peer-review

140 Citations (Scopus)

586 Downloads (Pure)

Abstract

A gene-directed chemical communication pathway between synthetic protocell signaling transmitters (lipid vesicles) and receivers (proteinosomes) was designed, built and tested using a bottom-up modular approach comprising small molecule transcriptional control, cell-free gene expression, porin-directed efflux, substrate signaling, and enzyme cascade-mediated processing.

Original language	English
Pages (from-to)	339-346
Number of pages	8
Journal	ACS Synthetic Biology
Volume	7
Issue number	2
Early online date	1 Nov 2017
DOIs	https://doi.org/10.1021/acssynbio.7b00306
Publication status	Published - 16 Feb 2018

Research Groups and Themes

BrisSynBio
Bristol BioDesign Institute

Keywords

cell-free expression
communication
enzyme cascade
genetic switches
lipid vesicles
proteinosomes
α-hemolysin

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10.1021/acssynbio.7b00306

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This is the author accepted manuscript (AAM). The final published version (version of record) is available online via ACS at https://pubs.acs.org/doi/full/10.1021/acssynbio.7b00306 . Please refer to any applicable terms of use of the publisher.
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AU - Tang, T. Y.Dora

AU - Cecchi, Dario

AU - Fracasso, Giorgio

AU - Accardi, Davide

AU - Coutable-Pennarun, Angelique

AU - Mansy, Sheref S.

AU - Perriman, Adam W.

AU - Anderson, J. L.Ross

AU - Mann, Stephen

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KW - communication

KW - enzyme cascade

KW - genetic switches

KW - lipid vesicles

KW - proteinosomes

KW - α-hemolysin

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JF - ACS Synthetic Biology

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ER -

Gene-Mediated Chemical Communication in Synthetic Protocell Communities

Abstract

Research Groups and Themes

Keywords

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Functional Biomolecular Liquids

Cite this

Gene-Mediated Chemical Communication in Synthetic Protocell Communities

Abstract

Research Groups and Themes

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Projects

Functional Biomolecular Liquids

Cite this