Gene transfer strategies for the physiologist

L F Wong, S Wells, D Murphy

    Research output: Contribution to journalArticle (Academic Journal)

    5 Citations (Scopus)

    Abstract

    Foreign genes can be introduced into whole animals using methods of germline transgenesis and somatic gene delivery. While germline transgenesis can generate useful animal models for genetic studies, it can be costly, time-consuming and requires the use of a large number of animals. An alternative means of gene transfer is to deliver genes to somatic cells using non-viral and viral technologies. Non-viral methods such as naked DNA injection, electroporation and liposome/cation lipid-mediated gene transfer are relatively inefficient. In contrast, viruses are effective vehicles that carry foreign genes into a cell rapidly and efficiently. Here we illustrate the usefulness of adenoviral vectors to express a potent and specific inhibitor of cAMP-dependent protein kinase (PKA) to study the role of cyclic 3',5'-cyclic AMP (cAMP) in the osmotic regulation of the vasopressin gene in a transgenic rat model. The ability to modify endogenous systems within specific cells in a whole animal model allows gene effects to be studied with physiological relevance. The combination of molecular biology and integrative physiology is a powerful application that can aid in the elucidation of how gene function can translate into complex systems in an organism.

    Original languageEnglish
    Pages (from-to)735-45
    Number of pages11
    JournalExperimental Physiology
    Volume85
    Issue number6
    Publication statusPublished - Nov 2000

    Keywords

    • Adenoviridae
    • Animals
    • Animals, Genetically Modified
    • Gene Transfer Techniques
    • Genetic Vectors
    • Germ Cells
    • Physiology

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