Abstract
We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure–function studies of novel eukaryotic complexes using single-molecule Förster resonance energy transfer as well as site-specific crosslinking strategies.
Original language | English |
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Pages (from-to) | 997–1000 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 13 |
Issue number | 12 |
Early online date | 17 Oct 2016 |
DOIs | |
Publication status | Published - Dec 2016 |
Keywords
- Biophysical chemistry
- Chemical tools
- Genetic engineering
- Protein design
- Proteins
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Professor Imre Berger
- School of Biochemistry - Professor of Biochemistry
- Infection and Immunity
Person: Academic , Member