Genetic engineering of Streptomyces coelicolor A3(2) for the enantioselective reduction of unnatural β-keto-ester substrates

Christopher E. Anson, Maureen J. Bibb, Kevin I. Booker-Milburn*, Cole Clissold, Patrick J. Haley, David A. Hopwood, Koji Ichinose, W. Peter Revill, G. Richard Stephenson, Chandres M. Surti

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

20 Citations (Scopus)

Abstract

Potential 'reagents' for the enantioselective reduction, and other biotransformations, of β-keto-esters result from the genetic engineering of Streptomyces coelicolor A3(2). For example, incubation of the N- acetylcysteamine thioester 1 with the recombinant strain CH999/pIJ5675 followed by treatment with MeOH/HCl gave the lactone 2 as essentially a single enantiomer.

Original languageEnglish
Pages (from-to)224-227
Number of pages4
JournalAngewandte Chemie - International Edition
Volume39
Issue number1
DOIs
Publication statusPublished - 3 Jan 2000

Keywords

  • Biosynthesis
  • Enzyme catalysis
  • Genetic engineering
  • Polyketides
  • Reductions

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