Potential 'reagents' for the enantioselective reduction, and other biotransformations, of β-keto-esters result from the genetic engineering of Streptomyces coelicolor A3(2). For example, incubation of the N- acetylcysteamine thioester 1 with the recombinant strain CH999/pIJ5675 followed by treatment with MeOH/HCl gave the lactone 2 as essentially a single enantiomer.
|Number of pages||4|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 3 Jan 2000|
- Enzyme catalysis
- Genetic engineering