Genome Wide Association Studies Identify CHRNA5/3 and HTR4 in the Development of Airflow Obstruction

Jemma B Wilk, Nick R G Shrine, Laura R Loehr, Jing Hua Zhao, Ani Manichaikul, Lorna M Lopez, Albert Vernon Smith, Susan R Heckbert, Joanna Smolonska, Wenbo Tang, Daan W Loth, Ivan Curjuric, Jennie Hui, Michael H Cho, Jeanne C Latourelle, Amanda P Henry, Melinda Aldrich, Per Bakke, Terri H Beaty, Amy R BentleyIngrid B Borecki, Guy G Brusselle, Kristin M Burkart, Ting-Hsu Chen, David Couper, James D Crapo, Gail Davies, Josee Dupuis, Nora Franceschini, Amund Gulsvik, Dana B Hancock, Tamara B Harris, Albert Hofman, Medea Imboden, Alan L James, Kay-Tee Khaw, Lies Lahousse, Lenore J Launer, Augusto Litonjua, Yongmei Liu, Kurt K Lohman, David A Lomas, Thomas Lumley, Kristin D Marciante, Wendy L McArdle, Bernd Meibohm, Alanna C Morrison, Arthur W Musk, Richard H Myers, Kari E North, Dirkje S Postma, Bruce M Psaty, Stephen S Rich, Fernando Rivadeneira, Thierry Rochat, Jerome I Rotter, María Soler Artigas, John M Starr, Andre G Uitterlinden, Nicholas J Wareham, Cisca Wijmenga, Pieter Zanen, Michael A Province, Edwin K Silverman, Ian J Deary, Lyle J Palmer, Patricia A Cassano, Vilmundur Gudnason, R Graham Barr, Ruth J F Loos, David P Strachan, Stephanie J London, H Marike Boezen, Nicole Probst-Hensch, Sina A Gharib, Ian P Hall, George T O'Connor, Martin D Tobin, Bruno H Stricker

Research output: Contribution to journalArticle (Academic Journal)peer-review

138 Citations (Scopus)


RATIONALE: Genome-wide association studies (GWAS) have identified loci influencing lung function, but fewer genes influencing chronic obstructive pulmonary disease (COPD) are known. OBJECTIVES: Perform meta-analyses of GWAS for airflow obstruction, a key pathophysiologic characteristic of COPD assessed by spirometry, in population-based cohorts examining all participants, ever smokers, never smokers, asthma free participants, and more severe cases. METHODS: Fifteen cohorts were studied for discovery (3,368 affected; 29,507 unaffected), and a population-based family study and a meta-analysis of case-control studies were used for replication and regional follow-up (3,837 cases; 4,479 controls). Airflow obstruction was defined as forced expiratory volume in one second (FEV(1)) and its ratio to forced vital capacity (FEV(1)/FVC) both less than their respective lower limits of normal as determined by published reference equations. MEASUREMENTS AND MAIN RESULTS: The discovery meta-analyses identified one region on chromosome 15q25.1 meeting genome-wide significance in ever smokers that includes AGPHD1, IREB2, and CHRNA5/CHRNA3 genes. The region was also modestly associated among never smokers. Gene expression studies confirmed the presence of CHRNA5/3 in lung, airway smooth muscle, and bronchial epithelial cells. A SNP in HTR4, a gene previously related to FEV(1)/FVC, achieved genome-wide statistical significance in combined meta-analysis. Top SNPs in ADAM19, RARB, PPAP2B, and ADAMTS19 were nominally replicated in the COPD meta-analysis. CONCLUSIONS: These results suggest an important role for the CHRNA5/3 region as a genetic risk factor for airflow obstruction that may be independent of smoking and implicate the HTR4 gene in the etiology of airflow obstruction.
Original languageEnglish
JournalAmerican Journal of Respiratory and Critical Care Medicine
Publication statusPublished - 2012


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