Genotyping coronaviruses associated with feline infectious peritonitis

Catherine S. Lewis, Emily Porter, David Matthews, Anja Kipar, Séverine Tasker, Christopher R. Helps, Stuart G. Siddell*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

16 Citations (Scopus)
396 Downloads (Pure)

Abstract

Feline coronavirus (FCoV) infections are endemic among cats worldwide. The majority of infections are asymptomatic or result in only mild enteric disease. However, approximately 5% of cases develop feline infectious peritonitis (FIP), a systemic disease that is a frequent cause of death in young cats. In this study, we report the complete coding genome sequences of six FCoVs: three from faecal samples from healthy cats and three from tissue lesion samples from cats with confirmed FIP. The six samples were obtained over a period of 8 weeks at a single-site cat rescue and rehoming centre in the UK. We found amino acid differences located at 44 positions across an alignment of the six virus translatomes and, at 21 of these positions, the differences fully or partially discriminated between the genomes derived from the faecal samples and the genomes derived from the tissue lesion samples. In this study, two amino acid differences fully discriminated the two classes of genomes: these were both located in the S2 domain of the virus surface glycoprotein gene. We also identified deletions in the 3c protein ORF of genomes from two of the FIP samples. Our results support previous studies that implicate S protein mutations in the pathogenesis of FIP.

Original languageEnglish
Pages (from-to)1358-1368
Number of pages11
JournalJournal of General Virology
Volume96
DOIs
Publication statusPublished - 1 Jun 2015

Bibliographical note

Date of Acceptance: 03/02/2015

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