GISP: a novel brain-specific protein that promotes surface expression and function of GABAB receptors

S Kantamneni, SA Correa, GK Hodgkinson, GG Meyer, NN Vinh, JM Henley, A Nishimune

Research output: Contribution to journalArticle (Academic Journal)peer-review

27 Citations (Scopus)

Abstract

Synaptic transmission depends on the regulated surface expression of neurotransmitter receptors, but many of the cellular processes required to achieve this remain poorly understood. To better define specific mechanisms for the GABAB receptor (GABABR) trafficking, we screened for proteins that bind to the carboxy-terminus of the GABAB1 subunit. We report the identification and characterization of a novel 130-kDa protein, GPCR interacting scaffolding protein (GISP), that interacts directly with the GABAB1 subunit via a coiled-coil domain. GISP co-fractionates with GABABR and with the postsynaptic density and co-immunoprecipitates with GABAB1 and GABAB2 from rat brain. In cultured hippocampal neurons, GISP displays a punctate dendritic distribution and has an overlapping localization with GABABRs. When co-expressed with GABABRs in human embryonic kidney cells, GISP promotes GABABR surface expression and enhances both baclofen-evoked extracellular signal-regulated kinase (ERK) phosphorylation and G-protein inwardly rectifying potassium channel (GIRK) currents. These results suggest that GISP is involved in the forward trafficking and stabilization of functional GABABRs.
Translated title of the contributionGISP: a novel brain-specific protein that promotes surface expression and function of GABAB receptors
Original languageEnglish
Pages (from-to)1003 - 1007
Number of pages5
JournalJournal of Neurochemistry
Volume100 (4)
DOIs
Publication statusPublished - Feb 2007

Bibliographical note

Publisher: Blackwell

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