Glucocorticoid treatment in patients with newly diagnosed immune thrombocytopenia switches CD14 ++CD16 + intermediate monocytes from a pro-inflammatory to an anti-inflammatory phenotype

Emily L Williams*, Madeleine L Stimpson, Philippa J P Lait, Lauren P Schewitz-Bowers, Lauren Jones, Ashwin Dhanda, Richard W J Lee, Charlotte A Bradbury

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)
13 Downloads (Pure)

Abstract

Immune thrombocytopenia (ITP) is thought to result from an aberrant adaptive autoimmune response, involving autoantibodies, B and T lymphocytes, directed at platelets and megakaryocytes. Previous reports have demonstrated skewed CD4+ T helper subset distribution and enhanced production of pro-inflammatory cytokines such as IL-17A and IFN-γ. The role of monocytes in ITP is less widely described, but innate immune cells have a role in shaping CD4+ T cell phenotypes. Glucocorticoids (GCs) are commonly used for first line ITP treatment and modulate a broad range of immune cells including T cells and monocytes. Using multiparameter flow cytometry analysis, we demonstrate expansion of intermediate monocytes (CD14++CD16+) in untreated newly diagnosed, ITP patients, with these cells displaying a pro-inflammatory phenotype, characterised by enhanced expression of CD64 and CD80. After 2 weeks of prednisolone treatment (1mg/kg daily), the proportion of intermediate monocytes reduced, with enhanced expression of the anti-inflammatory markers CD206 and CD163. Healthy control monocytes were distinctly different from those from ITP patients before and following GC-treatment. Furthermore, the GC-induced phenotype was not observed in patients with chronic ITP receiving thrombopoietin receptor agonists. These data suggest a role of monocytes in ITP pathogenesis and clinical response to GC-therapy.
Original languageEnglish
Pages (from-to)375-384
Number of pages10
JournalBritish Journal of Haematology
Volume192
Issue number2
Early online date18 Dec 2020
DOIs
Publication statusPublished - 18 Jan 2021

Keywords

  • autoimmunity
  • glucorticoids
  • steroids
  • immune thrombocytopenia
  • monocyte subsets

Fingerprint Dive into the research topics of 'Glucocorticoid treatment in patients with newly diagnosed immune thrombocytopenia switches CD14 <sup>++</sup>CD16 <sup>+</sup> intermediate monocytes from a pro-inflammatory to an anti-inflammatory phenotype'. Together they form a unique fingerprint.

Cite this