Glutamate and aspartate activated channels and inhibitory synaptic currents in large cerebellar neurons grown in culture

S G Cull-Candy, M M Usowicz

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)

Abstract

Patch-clamp methods were used to examine large (greater than 30 microns) cerebellar neurons of the rat, maintained in cell culture. Cells possessed voltage-activated transient inward Na+ currents which were sensitive to tetrodotoxin. Spontaneous synaptic currents, present in whole-cell recordings, were abolished by bicuculline and picrotoxin and were carried by Cl-. Cells produced inward currents in response to the transmitter candidates glutamate and aspartate and also to the glutamate agonists kainate, quisqualate, N-methyl-D-aspartate and ibotenate. Analysis of glutamate and aspartate-current noise has been used to derive characteristics of the excitatory channels. Single channel currents have also been observed directly in whole-cell and outside-out patches. Both glutamate and aspartate are able to activate channels which were blocked by Mg2+ and had a maximum conductance of 50 pS.

Original languageEnglish
Pages (from-to)182-7
Number of pages6
JournalBrain Research
Volume402
Issue number1
Publication statusPublished - 27 Jan 1987

Keywords

  • Animals
  • Aspartic Acid
  • Cerebellum
  • Culture Techniques
  • Glutamates
  • Glutamic Acid
  • Ion Channels
  • Membrane Potentials
  • Neural Inhibition
  • Rats
  • Synaptic Membranes
  • Journal Article
  • Research Support, Non-U.S. Gov't

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