Heavy Metal Resistance Genes Are Associated with blaNDM-1- and blaCTX-M-15-Carrying Enterobacteriaceae

Qiu E Yang; Siham Rajab Agouri; Jonathan Mark Tyrrell; Timothy Rutland Walsh

doi:10.1128/AAC.02642-17

Heavy Metal Resistance Genes Are Associated with blaNDM-1- and blaCTX-M-15-Carrying Enterobacteriaceae

Qiu E Yang, Siham Rajab Agouri, Jonathan Mark Tyrrell, Timothy Rutland Walsh

School of Cellular and Molecular Medicine

Research output: Contribution to journal › Article (Academic Journal) › peer-review

34 Citations (Scopus)

Abstract

The occurrence of heavy metal resistance genes in multiresistant Enterobacteriaceae possessing blaNDM-1 or blaCTX-M-15 genes was examined by PCR and pulsed-field gel electrophoresis with S1 nuclease. Compared with clinical susceptible isolates (10.0% to 30.0%), the pcoA, merA, silC, and arsA genes occurred with higher frequencies in blaNDM-1-positive (48.8% to 71.8%) and blaCTX-M-15-positive (19.4% to 52.8%) isolates, and they were mostly located on plasmids. Given the high association of metal resistance genes with multidrug-resistant Enterobacteriaceae, increased vigilance needs to be taken with the use of heavy metals in hospitals and the environment.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	5
DOIs	https://doi.org/10.1128/AAC.02642-17
Publication status	Published - May 2018

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Keywords

Anti-Bacterial Agents/pharmacology
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Enterobacteriaceae/drug effects
Metals, Heavy/pharmacology
Microbial Sensitivity Tests
Plasmids/genetics
beta-Lactamases/genetics

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title = "Heavy Metal Resistance Genes Are Associated with blaNDM-1- and blaCTX-M-15-Carrying Enterobacteriaceae",

abstract = "The occurrence of heavy metal resistance genes in multiresistant Enterobacteriaceae possessing blaNDM-1 or blaCTX-M-15 genes was examined by PCR and pulsed-field gel electrophoresis with S1 nuclease. Compared with clinical susceptible isolates (10.0% to 30.0%), the pcoA, merA, silC, and arsA genes occurred with higher frequencies in blaNDM-1-positive (48.8% to 71.8%) and blaCTX-M-15-positive (19.4% to 52.8%) isolates, and they were mostly located on plasmids. Given the high association of metal resistance genes with multidrug-resistant Enterobacteriaceae, increased vigilance needs to be taken with the use of heavy metals in hospitals and the environment.",

keywords = "Anti-Bacterial Agents/pharmacology, Drug Resistance, Bacterial, Electrophoresis, Gel, Pulsed-Field, Enterobacteriaceae/drug effects, Metals, Heavy/pharmacology, Microbial Sensitivity Tests, Plasmids/genetics, beta-Lactamases/genetics",

author = "Yang, {Qiu E} and Agouri, {Siham Rajab} and Tyrrell, {Jonathan Mark} and Walsh, {Timothy Rutland}",

note = "Copyright {\textcopyright} 2018 Yang et al.",

year = "2018",

month = may,

doi = "10.1128/AAC.02642-17",

language = "English",

volume = "62",

journal = "Antimicrobial Agents and Chemotherapy",

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publisher = "American Society for Microbiology",

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T1 - Heavy Metal Resistance Genes Are Associated with blaNDM-1- and blaCTX-M-15-Carrying Enterobacteriaceae

AU - Yang, Qiu E

AU - Agouri, Siham Rajab

AU - Tyrrell, Jonathan Mark

AU - Walsh, Timothy Rutland

PY - 2018/5

Y1 - 2018/5

N2 - The occurrence of heavy metal resistance genes in multiresistant Enterobacteriaceae possessing blaNDM-1 or blaCTX-M-15 genes was examined by PCR and pulsed-field gel electrophoresis with S1 nuclease. Compared with clinical susceptible isolates (10.0% to 30.0%), the pcoA, merA, silC, and arsA genes occurred with higher frequencies in blaNDM-1-positive (48.8% to 71.8%) and blaCTX-M-15-positive (19.4% to 52.8%) isolates, and they were mostly located on plasmids. Given the high association of metal resistance genes with multidrug-resistant Enterobacteriaceae, increased vigilance needs to be taken with the use of heavy metals in hospitals and the environment.

AB - The occurrence of heavy metal resistance genes in multiresistant Enterobacteriaceae possessing blaNDM-1 or blaCTX-M-15 genes was examined by PCR and pulsed-field gel electrophoresis with S1 nuclease. Compared with clinical susceptible isolates (10.0% to 30.0%), the pcoA, merA, silC, and arsA genes occurred with higher frequencies in blaNDM-1-positive (48.8% to 71.8%) and blaCTX-M-15-positive (19.4% to 52.8%) isolates, and they were mostly located on plasmids. Given the high association of metal resistance genes with multidrug-resistant Enterobacteriaceae, increased vigilance needs to be taken with the use of heavy metals in hospitals and the environment.

KW - Anti-Bacterial Agents/pharmacology

KW - Drug Resistance, Bacterial

KW - Electrophoresis, Gel, Pulsed-Field

KW - Enterobacteriaceae/drug effects

KW - Metals, Heavy/pharmacology

KW - Microbial Sensitivity Tests

KW - Plasmids/genetics

KW - beta-Lactamases/genetics

U2 - 10.1128/AAC.02642-17

DO - 10.1128/AAC.02642-17

M3 - Article (Academic Journal)

C2 - 29507071

SN - 0066-4804

VL - 62

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

ER -

Heavy Metal Resistance Genes Are Associated with blaNDM-1- and blaCTX-M-15-Carrying Enterobacteriaceae

Abstract

Bibliographical note

Keywords

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