Hemizona assay and sperm penetration assay in the prediction of IVF outcome: a systematic review

Paraskevi Vogiatzi, Charalampos Chrelias, David J Cahill, Maria Creatsa, Nikos Vrachnis, Zoe Iliodromiti, Demetrios Kassanos, Charalampos Siristatidis

Research output: Contribution to journalArticle (Academic Journal)peer-review

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The limited predictive value of semen analysis in achieving natural conception or in IVF outcome confirms the need for sperm function tests to determine optimal management. We reviewed HZA and SPA predictive power in IVF outcome, with statistical significance of diagnostic power of the assays. HZA was readily efficient in predicting IVF outcome, while evident inconsistency among the studies analysed framed the SPA's role in male fertility evaluation. Considerable variation was noted in the diagnostic accuracy values of SPA with wide sensitivity (52-100%), specificity (0-100%), and PPV (18-100%) and NPV (0-100%) together with fluctuation and notable differentiation in methodology and cutoff values employed by each group. HZA methodology was overall consistent with minor variation in cutoff values and oocyte source, while data analysis reported strong correlation between HZA results with IVF outcome, high sensitivity (75-100%), good specificity (57-100%), and high PPV (79-100%) and NPV (68-100%). HZA correlated well with IVF outcome and demonstrated better sensitivity/specificity and positive/negative predictive power. Males with normal or slightly abnormal semen profiles could benefit by this intervention and could be evaluated prior to referral to assisted reproduction. HZA should be used in a sequential fashion with semen analysis and potentially other bioassays in an IVF setting.

Original languageEnglish
Article number945825
Number of pages10
JournalBioMed Research International
Publication statusPublished - 19 Nov 2013


  • Female
  • Fertilization in Vitro
  • Humans
  • Male
  • Predictive Value of Tests
  • Pregnancy
  • Semen Analysis
  • Sperm-Ovum Interactions
  • Treatment Outcome
  • Zona Pellucida


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