Herpes simplex virus type 2 US3 blocks apoptosis induced by sorbitol treatment

Takayuki Murata, Fumi Goshima, Yohei Yamauchi, Tetsuo Koshizuka, Hiroki Takakuwa, Yukihiro Nishiyama

Research output: Contribution to journalArticle (Academic Journal)peer-review

19 Citations (Scopus)


Previously, we established HEp2 cell lines which express the US3 protein kinase of herpes simplex virus type 2 upon induction with IPTG. Using these cells, we examined whether expression of US3 is sufficient to protect cells from apoptotic cell death induced by sorbitol. Cells expressing US3 showed significantly reduced nuclear fragmentation in the degree that DNA fragmentation and caspase-3 activation were suppressed. It is known that stressors such as osmotic shock and UV irradiation induce the activation of the JNK (c-Jun N-terminal kinase), which can lead to apoptotic cell death. Expression of US3 resulted in the suppression of sorbitol-induced phosphorylation of JNK and MKK4/SEK1, suggesting that the suppression of apoptotic cell death was due to the attenuation of JNK activity.

Original languageEnglish
Pages (from-to)707-12
Number of pages6
JournalMicrobes and Infection
Issue number7
Publication statusPublished - Jun 2002


  • Apoptosis
  • Caspase 3
  • Caspases
  • Cell Line
  • Herpesvirus 2, Human
  • Humans
  • Isopropyl Thiogalactoside
  • JNK Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4
  • Mitogen-Activated Protein Kinase Kinases
  • Mitogen-Activated Protein Kinases
  • Phosphorylation
  • Protein-Serine-Threonine Kinases
  • Signal Transduction
  • Sorbitol
  • Viral Proteins
  • Journal Article
  • Research Support, Non-U.S. Gov't

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