High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis

Sheng Han, Marco Raabe, Lorna Hodgson, Judith Mantell, Paul Verkade, Theo Lasser, Katharina Landfester, Tanja Weil*, Ingo Lieberwirth

*Corresponding author for this work

Research output: Contribution to journalLetter (Academic Journal)

3 Citations (Scopus)
154 Downloads (Pure)

Abstract

Fluorescent nanodiamonds (fNDs) represent an emerging class of nanomaterials offering great opportunities for ultrahigh resolution imaging, sensing and drug delivery applications. Their biocompatibility, exceptional chemical and consistent photostability renders them particularly attractive for correlative light-electron microscopy studies providing unique insights into nanoparticle-cell interactions. Herein, we demonstrate a stringent procedure to image and quantify fNDs with a high contrast down to the single particle level in cells. Individual fNDs were directly visualized by energy-filtered transmission electron microscopy, that is, inside newly forming, early endosomal vesicles during their cellular uptake processes as well as inside cellular organelles such as a mitochondrion. Furthermore, we demonstrate the unequivocal identification, localization, and quantification of individual fNDs in larger fND clusters inside intracellular vesicles. Our studies are of great relevance to obtain quantitative information on nanoparticle trafficking and their various interactions with cells, membranes, and organelles, which will be crucial to design-improved sensors, imaging probes, and nanotherapeutics based on quantitative data.

Original languageEnglish
Pages (from-to)2178-2185
Number of pages8
JournalNano Letters
Volume19
Issue number3
Early online date26 Feb 2019
DOIs
Publication statusPublished - 13 Mar 2019

Keywords

  • Correlative light electron microscopy
  • energy-filtered transmission electron microscopy
  • nanodiamond
  • nanoparticle quantification
  • particle-cell interactions

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