High-Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates

Giordano Amoruso; Juntai Liu; Daniel W Polak; Kavita Tiwari; Michael R  Jones; Thomas A A Oliver

doi:10.1021/acs.jpclett.1c01407

High-Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates

Giordano Amoruso, Juntai Liu, Daniel W Polak, Kavita Tiwari, Michael R Jones ^*, Thomas A A Oliver^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

10 Citations (Scopus)

83 Downloads (Pure)

Abstract

Reaction centers (RCs) are the pivotal component of natural photosystems, converting solar energy into the potential difference between separated electrons and holes that is used to power much of biology. RCs from anoxygenic purple photosynthetic bacteria such as Rhodobacter sphaeroides only weakly absorb much of the visible region of the solar spectrum which limits their overall light-harvesting capacity. For in vitro applications such as bio-hybrid photodevices this deficiency can be addressed by effectively coupling RCs with synthetic light-harvesting materials. Here, we studied the time scale and efficiency of Förster resonance energy transfer (FRET) in a nanoconjugate assembled from a synthetic quantum dot (QD) antenna and a tailored RC engineered to be fluorescent. Time-correlated single photon counting spectroscopy of biohybrid conjugates enabled the direct determination of FRET from QDs to attached RCs on a time scale of 26.6 ± 0.1 ns and with a high efficiency of 0.75 ± 0.01.

Original language	English
Pages (from-to)	5448−5455
Number of pages	8
Journal	Journal of Physical Chemistry Letters
Volume	12
Issue number	23
Early online date	3 Jun 2021
DOIs	https://doi.org/10.1021/acs.jpclett.1c01407
Publication status	Published - 17 Jun 2021

Bibliographical note

Funding Information:
T.A.A.O. acknowledges financial support from the Royal Society for a Royal Society University Research Fellowship (UF1402310 and URF\R\201007) and a Research Fellows Enhancement Award (RGF\EA\180076). M.R.J., K.T., and J.L. acknowledge funding from the EPSRC/BBSRC Synthetic Biology Centre for Doctoral Training (EP/L016494/1) and from the BrisSynBio Synthetic Biology Research Centre at the University of Bristol (BB/L01386X/1). G.A. acknowledges EPSRC for a DTP Ph.D. studentship (EP/N509619/1).

Publisher Copyright:
© 2021 American Chemical Society.

Research Groups and Themes

BrisSynBio
Bristol BioDesign Institute

Keywords

Bacterial reaction centers
quantum dots
biohybrids
resonance-energy transfer
time-resolved spectroscopy
enhanced solar harvesting

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/acs.jpclett.1c01407

Full-text PDF (author’s accepted manuscript)
This is the author accepted manuscript (AAM). The final published version (version of record) is available online via American Chemical Society at https://pubs.acs.org/doi/10.1021/acs.jpclett.1c01407 . Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 1.67 MB

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Persistent link

8084 RS URF Renewal URF\R\201007. T Oliver
Oliver, T. (Principal Investigator)
1/10/20 → 30/09/23
Project: Research
8043 (CLOSED) BBSRC BrisSynBio M Jones
Jones, M. R. (Principal Investigator)
1/02/20 → 30/09/21
Project: Research
Elucidation of energy and charge transportation mechanisms in biomolecules and nanomaterials
Oliver, T. (Principal Investigator)
1/12/17 → 31/03/21
Project: Research

Ultrafast photoinduced bimolecular proton, electron and energy transfer
Amoruso, G. (Author), Oliver, T. (Supervisor) & Orr-Ewing, A. (Supervisor), 2 Dec 2021
Student thesis: Doctoral Thesis › Doctor of Philosophy (PhD)

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High Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates
Oliver, T. (Creator), Amoruso, G. (Creator) & Polak, D. (Creator), University of Bristol, 27 May 2021
DOI: 10.5523/bris.3hqsgdntr1g842l47xrke8duj0, http://data.bris.ac.uk/data/dataset/3hqsgdntr1g842l47xrke8duj0
Dataset

Cite this

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title = "High-Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates",

abstract = "Reaction centers (RCs) are the pivotal component of natural photosystems, converting solar energy into the potential difference between separated electrons and holes that is used to power much of biology. RCs from anoxygenic purple photosynthetic bacteria such as Rhodobacter sphaeroides only weakly absorb much of the visible region of the solar spectrum which limits their overall light-harvesting capacity. For in vitro applications such as bio-hybrid photodevices this deficiency can be addressed by effectively coupling RCs with synthetic light-harvesting materials. Here, we studied the time scale and efficiency of F{\"o}rster resonance energy transfer (FRET) in a nanoconjugate assembled from a synthetic quantum dot (QD) antenna and a tailored RC engineered to be fluorescent. Time-correlated single photon counting spectroscopy of biohybrid conjugates enabled the direct determination of FRET from QDs to attached RCs on a time scale of 26.6 ± 0.1 ns and with a high efficiency of 0.75 ± 0.01.",

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note = "Funding Information: T.A.A.O. acknowledges financial support from the Royal Society for a Royal Society University Research Fellowship (UF1402310 and URF\R\201007) and a Research Fellows Enhancement Award (RGF\EA\180076). M.R.J., K.T., and J.L. acknowledge funding from the EPSRC/BBSRC Synthetic Biology Centre for Doctoral Training (EP/L016494/1) and from the BrisSynBio Synthetic Biology Research Centre at the University of Bristol (BB/L01386X/1). G.A. acknowledges EPSRC for a DTP Ph.D. studentship (EP/N509619/1). Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

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AU - Jones , Michael R

AU - Oliver, Thomas A A

N1 - Funding Information: T.A.A.O. acknowledges financial support from the Royal Society for a Royal Society University Research Fellowship (UF1402310 and URF\R\201007) and a Research Fellows Enhancement Award (RGF\EA\180076). M.R.J., K.T., and J.L. acknowledge funding from the EPSRC/BBSRC Synthetic Biology Centre for Doctoral Training (EP/L016494/1) and from the BrisSynBio Synthetic Biology Research Centre at the University of Bristol (BB/L01386X/1). G.A. acknowledges EPSRC for a DTP Ph.D. studentship (EP/N509619/1). Publisher Copyright: © 2021 American Chemical Society.

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N2 - Reaction centers (RCs) are the pivotal component of natural photosystems, converting solar energy into the potential difference between separated electrons and holes that is used to power much of biology. RCs from anoxygenic purple photosynthetic bacteria such as Rhodobacter sphaeroides only weakly absorb much of the visible region of the solar spectrum which limits their overall light-harvesting capacity. For in vitro applications such as bio-hybrid photodevices this deficiency can be addressed by effectively coupling RCs with synthetic light-harvesting materials. Here, we studied the time scale and efficiency of Förster resonance energy transfer (FRET) in a nanoconjugate assembled from a synthetic quantum dot (QD) antenna and a tailored RC engineered to be fluorescent. Time-correlated single photon counting spectroscopy of biohybrid conjugates enabled the direct determination of FRET from QDs to attached RCs on a time scale of 26.6 ± 0.1 ns and with a high efficiency of 0.75 ± 0.01.

AB - Reaction centers (RCs) are the pivotal component of natural photosystems, converting solar energy into the potential difference between separated electrons and holes that is used to power much of biology. RCs from anoxygenic purple photosynthetic bacteria such as Rhodobacter sphaeroides only weakly absorb much of the visible region of the solar spectrum which limits their overall light-harvesting capacity. For in vitro applications such as bio-hybrid photodevices this deficiency can be addressed by effectively coupling RCs with synthetic light-harvesting materials. Here, we studied the time scale and efficiency of Förster resonance energy transfer (FRET) in a nanoconjugate assembled from a synthetic quantum dot (QD) antenna and a tailored RC engineered to be fluorescent. Time-correlated single photon counting spectroscopy of biohybrid conjugates enabled the direct determination of FRET from QDs to attached RCs on a time scale of 26.6 ± 0.1 ns and with a high efficiency of 0.75 ± 0.01.

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KW - quantum dots

KW - biohybrids

KW - resonance-energy transfer

KW - time-resolved spectroscopy

KW - enhanced solar harvesting

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SP - 5448−5455

JO - Journal of Physical Chemistry Letters

JF - Journal of Physical Chemistry Letters

IS - 23

ER -

High-Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates

Abstract

Bibliographical note

Research Groups and Themes

Keywords

UN SDGs

Access to Document

Handle.net

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Projects

8084 RS URF Renewal URF\R\201007. T Oliver

8043 (CLOSED) BBSRC BrisSynBio M Jones

Elucidation of energy and charge transportation mechanisms in biomolecules and nanomaterials

Student theses

Ultrafast photoinduced bimolecular proton, electron and energy transfer

Datasets

High Efficiency Excitation Energy Transfer in Biohybrid Quantum Dot–Bacterial Reaction Center Nanoconjugates

Cite this