High-fat diet blunts activation of the nuclear factor-κB signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats

Maria C Borges, Marco A R Vinolo, Amanda R Crisma, Ricardo A Fock, Primavera Borelli, Julio Tirapegui, Rui Curi, Marcelo M Rogero

Research output: Contribution to journalArticle (Academic Journal)peer-review

14 Citations (Scopus)

Abstract

OBJECTIVE: The present study was designed to investigate the effect of a high-fat diet (HFD) on the inflammatory response of peritoneal macrophages.

METHODS: Male Wistar rats were fed a control diet (n = 12) or an HFD (n = 12) for 12 wk. After euthanasia, peritoneal macrophages were collected and stimulated (or not) with lipopolysaccharide (LPS). Results from the assays using peritoneal macrophages were analyzed with one-way analysis of variance or an equivalent non-parametric test. The level of significance adopted was 0.05.

RESULTS: Consumption of the HFD was associated with significant increases in weight gain and fat depots (P < 0.05). Despite having no influence in systemic markers of inflammation, such as interleukin (IL)-6, tumor necrosis factor-α, and plasminogen activator inhibitor-1, the HFD intake significantly decreased insulin sensitivity, as evaluated by the homeostasis model assessment index (P < 0.05). A decreased production of IL-1β, IL-6, IL-10, and nitric oxide in response to the LPS stimulation was observed in peritoneal macrophages from the HFD group (P < 0.05). Also, in HFD-fed animals, LPS incubation did not increase IL-1β and IL-6 mRNA expression (P < 0.05). These effects were associated with an attenuation of IκB inhibitor kinase-β phosphorylation and nuclear factor-κB activation in response to LPS and with a failure to decrease IκB inhibitor-α expression (P < 0.05).

CONCLUSION: Chronic consumption of an HFD decreased the LPS-induced inflammatory response of peritoneal macrophages, which was associated with a downregulation of the nuclear factor-κB signaling pathway.

Original languageEnglish
Pages (from-to)443-9
Number of pages7
JournalNutrition
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 2013

Keywords

  • Animals
  • Biomarkers
  • Cells, Cultured
  • Diet, High-Fat
  • Down-Regulation
  • Inflammation
  • Insulin Resistance
  • Interleukin-10
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • Macrophages, Peritoneal
  • Male
  • NF-kappa B
  • Nitric Oxide
  • Phosphorylation
  • Plasminogen Activator Inhibitor 1
  • Rats
  • Rats, Wistar
  • Signal Transduction
  • Tumor Necrosis Factor-alpha
  • Weight Gain

Fingerprint

Dive into the research topics of 'High-fat diet blunts activation of the nuclear factor-κB signaling pathway in lipopolysaccharide-stimulated peritoneal macrophages of Wistar rats'. Together they form a unique fingerprint.

Cite this