Research output per year
Research output per year
Andrey Mikheikin, Anita Olsen, Loren Picco, Oliver Payton, Bud Mishra, James K. Gimzewski, Jason Reed*
Research output: Contribution to journal › Article (Academic Journal) › peer-review
Motivated by reports of low-level DNA contamination in popular commercial DNA purification kits, we employed a novel high-speed atomic force microscopy (HS-AFM) method to detect and characterize particulate and polymeric contaminants in four such systems: Qiagen MinElute PCR Purification, Zymo Research DNA Clean and Concentrator-5, Invitrogen ChargeSwitch-Pro PCR Purification, and Beckman Coulter AMPure XP. HS-AFM avoids amplification artifacts present in PCR or in the sequencing of amplified products, and it requires no chemical labels and easily achieves near-single-molecule sensitivity. Using this technique, we found trace levels of filamentous contamination, similar in appearance to dsDNA, in eluates from the Zymo, Qiagen, and ChargeSwitch kits. Conversely, we detected no contaminants in magnetic bead-based AMPure XP solutions. Eluates from the Zymo kits also tested positive for DNA in fluorescent intercalator dye and whole genome amplification (WGA) assays. Qiagen kits tested positive in the fluorescence assay but negative in the WGA assay. Both ChargeSwitch and AMPure XP tested negative in the fluorescence assay while the WGA results for these two kits were ambiguous. Taken together, our findings suggest AMPure XP would be the best choice for analyses requiring very high analytical stringency. While HS-AFM alone does not provide chemical specificity, it is a potentially valuable tool for characterizing and quantifying trace contaminants in molecular biology reagents and instruments in cases where conventional techniques fail.
Original language | English |
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Pages (from-to) | 2527-2532 |
Number of pages | 6 |
Journal | Analytical Chemistry |
Volume | 88 |
Issue number | 5 |
Early online date | 15 Feb 2016 |
DOIs | |
Publication status | Published - 1 Mar 2016 |
Research output: Contribution to journal › Article (Academic Journal) › peer-review
Scott, T. B. (Principal Investigator)
1/11/13 → 1/04/14
Project: Research