High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

Duygu Sari-Ak, Shervin Bahrami, Magdalena Laska, Petra Drncova, Daniel J Fitzgerald, Christiane Berger-Schaffitzel, Frederic Garzoni, Imre Berger

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to life attenuated or inactivated viruses for vaccination purposes. MultiBac is an advanced baculovirus expression vector system (BEVS) which consists of an engineered viral genome that can be customized for tailored applications. Here we describe the creation of a MultiBac-based VLP-factory™, based on the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.
Original languageEnglish
Title of host publicationHigh-Throughput Protein Production and Purification
Subtitle of host publicationMethods and Protocols
EditorsRenaud Vincentelli
PublisherHumana Press
Pages213-226
Number of pages14
ISBN (Electronic)978-1-4939-9624-7
ISBN (Print)978-1-4939-9623-0
DOIs
Publication statusE-pub ahead of print - 3 Jul 2019

Publication series

NameMethods in Molecular Biology
Volume2025
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Baculovirus expression vector system (BEVS)
  • Small-scale production
  • Virus-like particle (VLP)
  • MultiBac
  • Cre-LoxP fusion
  • Cre recombinase
  • Influenza
  • Hemagglutinin (HA)

Cite this

Sari-Ak, D., Bahrami, S., Laska, M., Drncova, P., Fitzgerald, D. J., Berger-Schaffitzel, C., Garzoni, F., & Berger, I. (2019). High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. In R. Vincentelli (Ed.), High-Throughput Protein Production and Purification: Methods and Protocols (pp. 213-226). (Methods in Molecular Biology; Vol. 2025). Humana Press. https://doi.org/10.1007/978-1-4939-9624-7_10