High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

Duygu Sari-Ak; Shervin Bahrami; Magdalena Laska; Petra Drncova; Daniel J Fitzgerald; Christiane Berger-Schaffitzel; Frederic Garzoni; Imre Berger

doi:10.1007/978-1-4939-9624-7_10

High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

Duygu Sari-Ak, Shervin Bahrami, Magdalena Laska, Petra Drncova, Daniel J Fitzgerald, Christiane Berger-Schaffitzel, Frederic Garzoni, Imre Berger

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

12 Citations (Scopus)

206 Downloads (Pure)

Abstract

Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to life attenuated or inactivated viruses for vaccination purposes. MultiBac is an advanced baculovirus expression vector system (BEVS) which consists of an engineered viral genome that can be customized for tailored applications. Here we describe the creation of a MultiBac-based VLP-factory™, based on the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.

Original language	English
Title of host publication	High-Throughput Protein Production and Purification
Subtitle of host publication	Methods and Protocols
Editors	Renaud Vincentelli
Publisher	Humana Press
Pages	213-226
Number of pages	14
ISBN (Electronic)	978-1-4939-9624-7
ISBN (Print)	978-1-4939-9623-0
DOIs	https://doi.org/10.1007/978-1-4939-9624-7_10
Publication status	E-pub ahead of print - 3 Jul 2019

Publication series

Name	Methods in Molecular Biology
Volume	2025
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Baculovirus expression vector system (BEVS)
Small-scale production
Virus-like particle (VLP)
MultiBac
Cre-LoxP fusion
Cre recombinase
Influenza
Hemagglutinin (HA)

Access to Document

10.1007/978-1-4939-9624-7_10

Full-text PDF (accepted author manuscript)
This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Springer NAture at https://link.springer.com/protocol/10.1007%2F978-1-4939-9624-7_10. Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 692 KB

Handle.net

Persistent link

Cite this

Sari-Ak, D., Bahrami, S., Laska, M., Drncova, P., Fitzgerald, D. J., Berger-Schaffitzel, C., Garzoni, F., & Berger, I. (2019). High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. In R. Vincentelli (Ed.), High-Throughput Protein Production and Purification: Methods and Protocols (pp. 213-226). (Methods in Molecular Biology; Vol. 2025). Humana Press. Advance online publication. https://doi.org/10.1007/978-1-4939-9624-7_10

Sari-Ak, Duygu ; Bahrami, Shervin ; Laska, Magdalena et al. / High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. High-Throughput Protein Production and Purification: Methods and Protocols. editor / Renaud Vincentelli. Humana Press, 2019. pp. 213-226 (Methods in Molecular Biology).

@inbook{ff6caff7bb9a4b9e814cdf2efb727b48,

title = "High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory{\texttrademark}, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression",

abstract = "Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to life attenuated or inactivated viruses for vaccination purposes. MultiBac is an advanced baculovirus expression vector system (BEVS) which consists of an engineered viral genome that can be customized for tailored applications. Here we describe the creation of a MultiBac-based VLP-factory{\texttrademark}, based on the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.",

keywords = "Baculovirus expression vector system (BEVS), Small-scale production, Virus-like particle (VLP), MultiBac, Cre-LoxP fusion, Cre recombinase, Influenza, Hemagglutinin (HA)",

author = "Duygu Sari-Ak and Shervin Bahrami and Magdalena Laska and Petra Drncova and Fitzgerald, \{Daniel J\} and Christiane Berger-Schaffitzel and Frederic Garzoni and Imre Berger",

year = "2019",

month = jul,

day = "3",

doi = "10.1007/978-1-4939-9624-7\_10",

language = "English",

isbn = "978-1-4939-9623-0",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "213--226",

editor = "Renaud Vincentelli",

booktitle = "High-Throughput Protein Production and Purification",

address = "United States",

}

Sari-Ak, D, Bahrami, S, Laska, M, Drncova, P, Fitzgerald, DJ, Berger-Schaffitzel, C, Garzoni, F & Berger, I 2019, High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. in R Vincentelli (ed.), High-Throughput Protein Production and Purification: Methods and Protocols. Methods in Molecular Biology, vol. 2025, Humana Press, pp. 213-226. https://doi.org/10.1007/978-1-4939-9624-7_10

High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. / Sari-Ak, Duygu; Bahrami, Shervin; Laska, Magdalena et al.
High-Throughput Protein Production and Purification: Methods and Protocols. ed. / Renaud Vincentelli. Humana Press, 2019. p. 213-226 (Methods in Molecular Biology; Vol. 2025).

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

TY - CHAP

T1 - High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

AU - Sari-Ak, Duygu

AU - Bahrami, Shervin

AU - Laska, Magdalena

AU - Drncova, Petra

AU - Fitzgerald, Daniel J

AU - Berger-Schaffitzel, Christiane

AU - Garzoni, Frederic

AU - Berger, Imre

PY - 2019/7/3

Y1 - 2019/7/3

N2 - Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to life attenuated or inactivated viruses for vaccination purposes. MultiBac is an advanced baculovirus expression vector system (BEVS) which consists of an engineered viral genome that can be customized for tailored applications. Here we describe the creation of a MultiBac-based VLP-factory™, based on the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.

AB - Baculovirus-based expression of proteins in insect cell cultures has emerged as a powerful technology to produce complex protein biologics for many applications ranging from multiprotein complex structural biology to manufacturing of therapeutic proteins including virus-like particles (VLPs). VLPs are protein assemblies that mimic live viruses but typically do not contain any genetic material, and therefore are safe and attractive alternatives to life attenuated or inactivated viruses for vaccination purposes. MultiBac is an advanced baculovirus expression vector system (BEVS) which consists of an engineered viral genome that can be customized for tailored applications. Here we describe the creation of a MultiBac-based VLP-factory™, based on the M1 capsid protein from influenza, and its application to produce in a parallelized fashion an array of influenza-derived VLPs containing functional mutations in influenza hemagglutinin (HA) thought to modulate the immune response elicited by the VLP.

KW - Baculovirus expression vector system (BEVS)

KW - Small-scale production

KW - Virus-like particle (VLP)

KW - MultiBac

KW - Cre-LoxP fusion

KW - Cre recombinase

KW - Influenza

KW - Hemagglutinin (HA)

U2 - 10.1007/978-1-4939-9624-7_10

DO - 10.1007/978-1-4939-9624-7_10

M3 - Chapter in a book

C2 - 31267455

SN - 978-1-4939-9623-0

T3 - Methods in Molecular Biology

SP - 213

EP - 226

BT - High-Throughput Protein Production and Purification

A2 - Vincentelli, Renaud

PB - Humana Press

ER -

Sari-Ak D, Bahrami S, Laska M, Drncova P, Fitzgerald DJ, Berger-Schaffitzel C et al. High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression. In Vincentelli R, editor, High-Throughput Protein Production and Purification: Methods and Protocols. Humana Press. 2019. p. 213-226. (Methods in Molecular Biology). Epub 2019 Jul 3. doi: 10.1007/978-1-4939-9624-7_10

High-Throughput Production of Influenza Virus-Like Particle (VLP) Array by Using VLP-factory™, a MultiBac Baculoviral Genome Customized for Enveloped VLP Expression

Abstract

Publication series

UN SDGs

Keywords

Access to Document

Handle.net

Fingerprint

Cite this