Highly efficient baculovirus-mediated multigene delivery in primary cells

Maysam Maysam Mansouri, Itxaso Bellon-Echeverria, Aurélien Rizk, Zahra Ehsaei, Chiara Cianciolo Cosentino, Catarina S. Silva, Imre Berger

Research output: Contribution to journalArticle (Academic Journal)peer-review

49 Citations (Scopus)
363 Downloads (Pure)

Abstract

Multigene delivery and subsequent cellular expression is emerging as a key technology required in diverse research fields including, synthetic and structural biology, cellular reprogramming and functional pharmaceutical screening. Current viral delivery systems such as retro- and adenoviruses suffer from limited DNA cargo capacity, thus impeding unrestricted multigene expression. We developed MultiPrime, a modular, non-cytotoxic, non-integrating, baculovirus-based vector system expediting highly efficient transient multigene expression from a variety of promoters. MultiPrime viruses efficiently transduce a wide range of cell types, including non-dividing primary neurons and induced-pluripotent stem cells (iPS). We show that MultiPrime can be used for reprogramming, and for genome editing and engineering by CRISPR/Cas9. Moreover, we implemented dual-host-specific cassettes enabling multiprotein expression in insect and mammalian cells using a single reagent. Our experiments establish MultiPrime as a powerful and highly efficient tool, to deliver multiple genes for a wide range of applications in primary and established mammalian cells.
Original languageEnglish
Article number11529 (2016)
Number of pages13
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 4 May 2016

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • SYNTHETIC BIOLOGY

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